TITLE

Purification of RNA from cells and tissues by acid phenol—guanidinium thiocyanate—chloroform extraction

PUB. DATE
February 2006
SOURCE
Nature Methods;Feb2006, Vol. 3 Issue 2, p149
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The article presents a protocol which describes a single-step technique for RNA purification. The cells or tissue samples are homogenized in guanidinium then RNA is purified from the lysate by extraction with phenol-chloroform at reduced pH. RNA yield depends on the tissue or cell source. The reagents used in the procedure were prepared with pyrocarbonate-treated water.
ACCESSION #
19504544

 

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