TITLE

NMR mapping of protein interactions in living cells

AUTHOR(S)
Selenko, Philipp; Wagner, Gerhard
PUB. DATE
February 2006
SOURCE
Nature Methods;Feb2006, Vol. 3 Issue 2, p80
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The article focuses on an application of in-cell nuclear magnetic resonance spectroscopy which analyzes the structural changes that go with protein-protein interactions in vivo and at atomic resolution. The attempts to develop new in vivo techniques for structural biology and cellular imaging aim to allow the study of biomolecules in their natural environment. Nuclear magnetic resonance spectroscopy is the only other method for the structural investigation of biomolecules at the atomic level, also in the structural investigation of labeled proteins in vivo and inside cells.
ACCESSION #
19504534

 

Related Articles

  • Mapping the binding site of full length HIV-1 Nef on human Lck SH3 by NMR spectroscopy. Briese, Lars; Preusser, Andrea; Willbold, Dieter // Journal of Biomedical Science;May2005, Vol. 12 Issue 3, p451 

    The Nef protein of human immunodeficiency virus type 1 (HIV-1) is known to directly bind to the SH3 domain of human lymphocyte specific kinase (Lck) via a proline-rich region located in the amino terminal part of Nef. To address the question whether Nef binding to Lck SH3 involves residues...

  • Carbonic Anhydrase B Interactions with Water and Urea. Prokhorov, D. A.; Kutyshenko, V. P.; Khristoforov, V. S. // Molecular Biology;May2005, Vol. 39 Issue 3, p438 

    High-resolution NMR spectroscopy has been used to study native carbonic anhydrase B unfolding with urea at pH 5.75 and T = 298 K. The rigidity parameter reflecting the effectiveness of spin diffusion (SD) displays a sigma-like dependence on urea concentration, which is characteristic of...

  • Phf19 links methylated Lys36 of histone H3 to regulation of Polycomb activity. Ballaré, Cecilia; Lange, Martin; Lapinaite, Audrone; Martin, Gloria Mas; Morey, Lluis; Pascual, Gloria; Liefke, Robert; Simon, Bernd; Shi, Yang; Gozani, Or; Carlomagno, Teresa; Benitah, Salvador Aznar; Di Croce, Luciano // Nature Structural & Molecular Biology;Dec2012, Vol. 19 Issue 12, p1257 

    Polycomb-group proteins are transcriptional repressors with essential roles in embryonic development. Polycomb repressive complex 2 (PRC2) contains the methyltransferase activity for Lys27. However, the role of other histone modifications in regulating PRC2 activity is just beginning to be...

  • Protein interaction patterns in different cellular environments are revealed by in-cell NMR. Barbieri, Letizia; Luchinat, Enrico; Banci, Lucia // Scientific Reports;9/25/2015, p14456 

    In-cell NMR allows obtaining atomic-level information on biological macromolecules in their physiological environment. Soluble proteins may interact with the cellular environment in different ways: either specifically, with their functional partners, or non-specifically, with other cellular...

  • Modeling cellular machinery through biological network comparison. Sharan, Roded; Ideker, Trey // Nature Biotechnology;Apr2006, Vol. 24 Issue 4, p427 

    Molecular networks represent the backbone of molecular activity within the cell. Recent studies have taken a comparative approach toward interpreting these networks, contrasting networks of different species and molecular types, and under varying conditions. In this review, we survey the field...

  • Direct detection of CH/Ï€ interactions in proteins. Plevin, Michael J.; Bryce, David L.; Boisbouvier, Jérôme // Nature Chemistry;Jun2010, Vol. 2 Issue 6, p466 

    XH/π interactions make important contributions to biomolecular structure and function. These weakly polar interactions, involving π-system acceptor groups, are usually identified from the three-dimensional structures of proteins. Here, nuclear magnetic resonance spectroscopy has been...

  • Cell wall teichoic acids fromStreptomyces daghestanicusVKM Ac-1722T andstreptomyces murinusINA-00524T. Streshinskaya, G.; Kozlova, Yu.; Alferova, I.; Shashkov, A.; Evtushenko, L. // Microbiology (00262617);Jan2005, Vol. 74 Issue 1, p40 

    The structure of cell wall teichoic acids was studied by chemical methods and NMR spectroscopy in the type strains of two actinomycete species of the“Streptomyces griseoviridis”phenetic cluster:streptomyces daghestanicusandstreptomyces murinus. S. daghestanicusVKM Ac-1722t contained...

  • A New Furanocoumarin from the Roots of Angelica oncosepala. Qing, Chen; Zhou, Yi-Ping; Zi, Rong; Zheng, Bin; Luo, Ming; Li, Xian // Chemistry of Natural Compounds;May2014, Vol. 50 Issue 2, p251 

    A new furanocoumarin, 5-(2″,3″-dihydroxy-3″-methylbutyloxy)-8-(2″′-hydroxy-3″′-methyl-3″′-butylenoxy)-psoralen, named oncosepalin B ( 1), was isolated from the roots of Angelica oncosepala. The structure of the new compound was identified on...

  • Effects of Toxins Pi2 and Pi3 on Human T Lymphocyte Kv1.3 Channels: The Role of Glu7 and Lys24. P�ter Jr., M.; Varga, Z.; Hajdu, P.; G�sp�r Jr., R.; Damjanovich, S.; Horjales, E.; Possani, L.D.; Panyi, G. // Journal of Membrane Biology;Jan2001, Vol. 179 Issue 1, p13 

    Pandinus imperator scorpion toxins Pi2 and Pi3 differ only by a single amino acid residue (neutral Pro7 in Pi2 vs. acidic Glu7 in Pi3). The binding kinetics of these toxins to human Kv1.3 showed that the decreased on rate (kON= 2.18 � 108m-1sec-1 for Pi2 and 1.28 � 107m-1sec-1 for Pi3)...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics