TITLE

Mapping structural interactions using in-cell NMR spectroscopy (STINT-NMR)

AUTHOR(S)
Burz, David S.; Dutta, Kaushik; Cowburn, David; Shekhtman, Alexander
PUB. DATE
February 2006
SOURCE
Nature Methods;Feb2006, Vol. 3 Issue 2, p91
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We describe a high-throughput in-cell nuclear magnetic resonance (NMR)-based method for mapping the structural changes that accompany protein-protein interactions (STINT-NMR). The method entails sequentially expressing two (or more) proteins within a single bacterial cell in a time-controlled manner and monitoring the protein interactions using in-cell NMR spectroscopy. The resulting spectra provide a complete titration of the interaction and define structural details of the interacting surfaces at atomic resolution.
ACCESSION #
19504527

 

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