TITLE

A multiphoton laser scanning microscope setup for transcranial in vivo brain imaging on mice

AUTHOR(S)
Nase, Gabriele; Helm, P. Johannes; Reppen, Trond; Ottersen, Ole Petter
PUB. DATE
December 2005
SOURCE
Review of Scientific Instruments;Dec2005, Vol. 76 Issue 12, p123702
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We describe a multiphoton laser scanning microscope setup for transcranial in vivo brain imaging in mice. The modular system is based on a modified industrial standard Confocal Scanning Laser Microscope (CSLM) and is assembled mainly from commercially available components. A special multifunctional stage, which is optimized for both laser scanning microscopic observation and preparative animal surgery, has been developed and built. The detection unit includes a highly efficient photomultiplier tube installed in a Peltier-cooled thermal box shielding the detector from changes in room temperature and from distortions caused by external electromagnetic fields. The images are recorded using a 12-bit analog–to–digital converter. Depending on the characteristics of the staining, individual nerve cells can be imaged down to at least 100 μm below the intact cranium and down to at least 200 μm below the opened cranium.
ACCESSION #
19421307

 

Related Articles

  • Simple pressure-tuned Fabry–Pérot interferometer. Hansis, E.; Cubel, T.; Choi, J.-H.; Guest, J. R.; Raithel, G. // Review of Scientific Instruments;Mar2005, Vol. 76 Issue 3, p033105 

    A simple, compact and inexpensive pressure-tuned Fabry–Pérot interferometer is presented. It is used as a laser locking reference for optical frequencies where the use of an atomic reference is impractical. The scanning range is several GHz. Absolute positioning of the interferometer...

  • 73-W-long pulse kinetically enhanced copper vapor laser using wide aperture discharge tube. Singh, B.; Subramaniam, V. V.; Daultabad, S. R.; Chakraborty, A. // Review of Scientific Instruments;Dec2005, Vol. 76 Issue 12, p126104 

    A wide aperture kinetically enhanced copper vapor laser consisting of a ∼47 mm bore and 1450 mm length discharge tube is demonstrated with moderate input power of ∼5 kW, and overall efficiency of ∼1.45%. The laser pulse durations were observed to be ∼60 ns full width at half...

  • Pulsed laser heating and temperature determination in a diamond anvil cell. Deemyad, S.; Sterer, E.; Barthel, C.; Rekhi, S.; Tempere, J.; Silvera, Isaac F. // Review of Scientific Instruments;Dec2005, Vol. 76 Issue 12, p125104 

    Pulsed laser heating in a diamond anvil cell has several advantages over cw heating: it can suppress thermally activated diffusion of the pressurization medium into the gasket and diamond, suppress chemical reactions of impurities with the sample and environment, requires far less average power...

  • Active clipping system for transversely exited CO2 lasers. Bellecci, C.; Gaudio, P.; Martellucci, S.; Penco, E.; Richetta, M. // Review of Scientific Instruments;Feb2005, Vol. 76 Issue 2, p026115 

    A simple intracavity system, based on electrical discharge, is used to control the shape and duration of transverse electric CO2 laser pulses. The clipping mechanism is based on active locking at the same frequency of the laser. The clipping apparatus can deliver gain switched spikes pulses...

  • Strait-Line Laser Level 120. Berendsohn, Roy; Derene, Glenn // Popular Mechanics;Jan2005, Vol. 182 Issue 1, p36 

    This article discusses the technical aspects associated with Strait-Line laser device. The appealingly simple Strait-Line attaches to the wall with 3M Command Strips. This level can swivel to shoot a line vertically, horizontally or at any angle. When shooting vertical lines, users need to aim...

  • Twin optical traps for two-particle cross-correlation measurements: Eliminating cross-talk. Atakhorrami, M.; Addas, K. M.; Schmidt, C. F. // Review of Scientific Instruments;Apr2008, Vol. 79 Issue 4, p043103 

    The correlated motions of two micron-sized particles reflect the (micro-) rheological properties of a fluid and can be conveniently detected using two optical traps in combination with interferometric displacement detection. When the correlations become small, cross-talk between the two beams...

  • Laser-launched flyer plates for shock physics experiments. Swift, Damian C.; Niemczura, Johnathan G.; Paisley, Dennis L.; Johnson, Randall P.; Sheng-Nian Luo; Tierney IV, Thomas E. // Review of Scientific Instruments;Sep2005, Vol. 76 Issue 9, p093907 

    The TRIDENT laser was used to launch Cu, Ga, and NiTi flyers from poly(methylmethacrylate) (PMMA) substrates, coated with thin (∼micron) layers to absorb the laser energy, confine the plasma, and insulate the flyer. The laser pulse was ∼600 ns long, and the flyers were 50 to 250 μm...

  • Single beam optical tweezers setup with backscattered light detection for three-dimensional measurements on DNA and nanopores. Sischka, Andy; Kleimann, Christoph; Hachmann, Wiebke; Schäfer, Marcus M.; Seuffert, Ina; Tönsing, Katja; Anselmetti, Dario // Review of Scientific Instruments;Jun2008, Vol. 79 Issue 6, p063702 

    We introduce a versatile and high precision three-dimensional optical tweezers setup with minimal optical interference to measure small forces and manipulate single molecules in the vicinity of a weak reflective surface. Our tweezers system integrates an inverted optical microscope with a single...

  • Laser Doppler velocimetry based on the photoacoustic effect in a CO2 laser. Choi, Jong-woon; Yu, Moon-jong; Kopica, Mirek; Woo, Sam-yong; Choi, Yong-Seok // Review of Scientific Instruments;Feb2005, Vol. 76 Issue 2, p023109 

    We report a simple laser Doppler velocimeter in which the photoacoustic effect was used to measure the rotation wheel speed. A Doppler signal, caused by mixing a returning wave with an originally existing wave inside the CO2 laser cavity, was detected using a microphone in the laser tube....

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics