Mutation in the Sp1 motif of the bovine leptin gene affects its expression

Adamowicz, Tatiana; Flisikowski, Krzysztof; Starzyński, Rafał; Zwierzchowski, Lech; Świtoński, Marek
January 2006
Mammalian Genome;Jan2006, Vol. 17 Issue 1, p77
Academic Journal
Leptin is expressed mainly by adipocytes and plays a crucial role in the regulation of energy expenditure, food intake, and adiposity. Using PCR-heteroduplex analysis and sequencing, we investigated a C/G substitution in the promoter region of the bovine leptin gene. Application of the electrophoretic mobility shift assay showed that the C→G transversion decreased the leptin gene promoter binding capacity for nuclear proteins. With real-time PCR and Western blotting, we showed that the leptin expression level was higher in cattle with the CC than with the GG genotype.


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