In vitro permeation of chromium species through porcine and human skin as determined by capillary electrophoresis–inductively coupled plasma–sector field mass spectrometry

Van Lierde, Veerle; Chéry, Cyrille C.; Roche, Nathalie; Monstrey, Stan; Moens, Luc; Vanhaecke, Frank
January 2006
Analytical & Bioanalytical Chemistry;Jan2006, Vol. 384 Issue 2, p378
Academic Journal
Since the species that trigger chromium allergy are not yet known, it is important to gain more of an insight into the mechanism of chromium transport through the skin and into the relationship between chromium allergy and chromium species. In vitro permeation studies with porcine and human skin were performed using a Franz static diffusion cell. Investigations attempted to elucidate (i) which Cr compounds are able to permeate through skin, (ii) the influence the Cr concentration in the donor solution has on the Cr permeation, and (iii) the effect that the time of exposure to the donor solution has on Cr permeation. Capillary electrophoresis hyphenated to inductively coupled plasma–sector field mass spectrometry (CE–ICP–SFMS) was used to separate and quantify the Cr species in the receptor fluid. 50 mmol L−1 phosphate buffer (pH 2.5) was used for CE separation, and two different electrophoretic runs were carried out (in the positive and negative modes). Pneumatic nebulization (PN)-ICP-SFMS was used in order to quantify the total amount of Cr absorbed by the skin after microwave-assisted acid digestion of the tissue. Cr(VI) was found to pass most easily through the skin. Nevertheless, Cr(VI) was also shown to be absorbed more efficiently by the skin than Cr(III), an observation attributed to a more pronounced rejection of the positively charged Cr(III) ions by the skin barrier. These results were in good agreement with in vitro permeation studies previously reported in the literature in which other analytical techniques were used. Differences observed in the permeation of Cr following the application of aqueous Cr donor solutions and Cr-containing simulated sweat donor solutions are also described. [Figure not available: see fulltext.]


Related Articles

  • On-column sample enrichment for the high-sensitivity sheath-flow CE-MS analysis of peptides. Sandra, K.; Lynen, F.; Devreese, B.; Van Beeumen, J.; Sandra, P. // Analytical & Bioanalytical Chemistry;Jul2006, Vol. 385 Issue 4, p671 

    A sample enrichment technique to increase sensitivity in capillary electrophoresis–mass spectrometry (CE-MS) is described. Peptides or glycopeptides are retained and concentrated on a short (3–5-mm) reversed-phase (C18) packed-bed situated in the fused-silica separation capillary...

  • Optimization of a rapid capillary electrophoresis ESI-IT tandem mass spectrometry method for the analysis of short-chain carnitines in human plasma. Desiderio, Claudia; De Rossi, Antonella; Inzitari, Rosanna; Mancinelli, Angelo; Rossetti, Diana Valeria; Castagnola, Massimo; Messana, Irene // Analytical & Bioanalytical Chemistry;Mar2008, Vol. 390 Issue 6, p1637 

    A capillary electrophoresis (CE) method coupled to electrospray ionization ion trap tandem mass spectrometry (ESI-IT-MS/MS) is described for the rapid analysis of carnitine, acetylcarnitine, and propionylcarnitine in human plasma. Optimization of the procedure was achieved by a reduced sample...

  • CE-ICP-MS: Advantages and Improvements in Selenium Speciation. Michalke, Bernhard // Spectroscopy;Apr2000, Vol. 15 Issue 4, p30 

    Presents information on a study which discussed the improvements of the capillary electrophoresis-inductively coupled plasma-mass spectrometry technique with respect to selenium speciation. Quality control aspects of the study; Time reduction, enhanced nebulization and improvement of...

  • CE--MS in Pharmaceutical Development and Medical Protein Research. Jimidar, M. Ilias // LC-GC Europe;Feb2004, Vol. 17 Issue 2, p112 

    Reports on the organization of the "Fifth Belgian CE-Users Group Meeting; Capillary Electrophoresis-Mass Spectrometry (CE-MS) in Pharmaceutical Development and Medical Protein Research," by the Belgian CE-Users group within the Royal Flemish Chemical Society (KVCV). Discussion of the status and...

  • Interactions of a novel ruthenium-based anticancer drug (KP1019 or FFC14a) with serum proteins - significance for the patient. Hartinger, C. G.; Hann, S.; Koellensperger, G.; Sulyok, M.; Groessl, M.; Timerbaev, A. R.; Rudnev, A. V.; Stingeder, G.; Keppler, B. K. // International Journal of Clinical Pharmacology & Therapeutics;2005, Vol. 43 Issue 12, p583 

    The article cites a research study that determines the efficacy of a novel ruthenium-based anticancer drug (KP1019 or FFC14a) with serum proteins. For the determination of binding constants and reaction kinetics of KP1019 with albumin and transferrin and to determine drug distribution between...

  • Molecular characterization and phylogenetic analysis of a novel glutenin gene (Dy10.1t) from Aegilops tauschii. Yanzhen Zhang; Qiaoyun Li; Yueming Yan; Jigang Zheng; Xueli An; Yinghua Xiao; Aili Wang; Yuhe Pei; Haibo Wang; Hsam, Sai L. K.; Zeller, Friedrich J. // Genome;Jul2006, Vol. 49 Issue 7, p735 

    A novel y-type high molecular mass glutenin subunit (HMM-GS) possessing a mobility that is slightly slower than that of the subunit Dy10 obtained by SDS–PAGE, named Dy10.1t, in the wild wheat Aegilops tauschii was identified by 1- and 2-dimensional gel electrophoresis, capillary...

  • Urinary proteome pattern in children with renal Fanconi syndrome. Drube, Jens; Schiffer, Eric; Mischak, Harald; Kemper, Markus J.; Neuhaus, Thomas; Pape, Lars; Lichtinghagen, Ralf; Ehrich, Jochen H. H. // Nephrology Dialysis Transplantation;Jul2009, Vol. 24 Issue 7, p2161 

    Background. The renal Fanconi syndrome (FS) is characterized by renal glucosuria, loss of electrolytes, bicarbonate and lactate, generalized hyperaminoaciduria and low-molecular-weight proteinuria. We studied the urinary low-molecular-weight proteome to identify excreted peptides indicative of a...

  • In-capillary micro solid-phase extraction and capillary electrophoresis separation of heterocyclic aromatic amines with nanospray mass spectrometric detection. Viberg, Peter; Nilsson, Staffan; Skog, Kerstin // Analytical & Bioanalytical Chemistry;Apr2004, Vol. 378 Issue 7, p1729 

    A miniaturised technique to analyse and detect heterocyclic aromatic amines (HAs) using micro solid-phase extraction (μSPE) coupled on-line (in-capillary) to capillary electrophoresis (CE) separation with nanospray (nESI) mass spectrometry (MS) detection has been developed. HAs are mutagenic...

  • Analysis of methotrexate and its eight metabolites in cerebrospinal fluid by solid-phase extraction and triple-stacking capillary electrophoresis. Hui-Ling Cheng; Shyh-Shin Chiou; Yu-Mei Liao; Chi-Yu Lu; Yen-Ling Chen; Shou-Mei Wu // Analytical & Bioanalytical Chemistry;Nov2010, Vol. 398 Issue 5, p2183 

    We establish a triple-stacking capillary electrophoresis (CE) separation method to monitor methotrexate (MTX) and its eight metabolites in cerebrospinal fluid (CSF). Three stacking methods with different mechanisms were combined and incorporated into CE separation. Complete stacking and sharp...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics