Production and application of amylase enzyme

Shukla, S. R.; Ananthanarayan, L.; Damle, Amey J.
November 2005
Colourage;Nov2005, Vol. 52 Issue 11, p43
Trade Publication
Production of amylase with corn starch as carbon source by Aspergillus niger ATCC 16404 is reported. In submerged cultivation system, various parameters were optimised. Maximum amylase activity was found after 4 days at pH 6 of media. The highest amylase production was obtained with 2% corn starch as a carbon source. The enzyme was purified from culture filtrate by ammonium salt precipitation method. Desizing of cotton sample with purified amylase was carried out and compared with commercial available amylase sample. Desizing of grey cotton fabric was carried out. However the efficiency of the synthesised amylase was found to be lower than a commercial sample. Possible remedies are suggested.


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