Eosinophil granulocytes are activated during the remission phase of ulcerative colitis

Lampinen, M.; Rönnblom, A.; Amin, K.; Kristjansson, G.; Rorsman, F.; Songfelt, P.; Säfsten, B.; Wagner, M.; Wanders, A.; Winqvist, O.; Carlson, M.
December 2005
Gut;Dec2005, Vol. 54 Issue 12, p1714
Academic Journal
Aim: The aim of this study was to establish a method of investigating intestinal eosinophil and neutrophil granulocytes by flow cytometry, and to compare the distribution and activity of these cells in different stages of ulcerative colitis (UC). Methods: Biopsy samples were taken from six locations of the entire colon and from the terminal ileum in 10 patients with active total UC, 10 patients with inactive total UC, eight patients with active distal UC, and 11 control subjects. Cell suspensions from biopsies and from peripheral blood were incubated with fluorophore conjugated monoclonal antibodies. The use of scatter plot-gating and specific antibodies was established in a flow cytometry assay. Results: Eosinophils were more numerous and more active in patients with active UC than in controls. Interestingly, during inactive UC, the number of activated eosinophils was even larger. Eosinophil activity was high in the rectum of patients with distal colitis but was also slightly elevated in the proximal colon. Neutrophils were increased in number and activity during active but not inactive UC. In patients with distal colitis, activated neutrophils were only found in the sigmoid colon and rectum. Conclusion: With this method, we confirm that neutrophils participate in the inflammatory process during active UC, and that they express a resting phenotype during remission. The finding of activated eosinophils in inflamed intestine strengthens the view of these cells as proinflammatory and tissue damaging. Nevertheless, our new finding of high eosinophil activation during inactive UC suggests that eosinophils play a role in repair of injured epithelium.


Related Articles

  • Neutrophil and Eosinophil Granule Proteins as Markers of Response to Local Prednisolone Treatment in Distal Ulcerative Colitis and Proctitis. Sangfelt, Per; Carlson, Marie; Thörn, Magnus; Lööf, Lars; Raab, Yngve // American Journal of Gastroenterology;Apr2001, Vol. 96 Issue 4, p1085 

    OBJECTIVE: The pathophysiological role of neutrophil and eosinophil granulocytes in relation to steroid enema treatment was studied in patients with distal ulcerative colitis and proctitis. METHODS: The rectal release of the neutrophil (myeloperoxidase, MPO), and eosinophil (eosinophilic...

  • Increased Intraluminal Release of Eosinophil Granule Proteins EPO, ECP, EPX, and Cytokines in Ulcerative Colitis and Proctitis in Segmental Perfusion. Carlson, Marie; Raab, Yngve; Peterson, Christer; Hällgren, Roger; Venge, Per // American Journal of Gastroenterology;Jul1999, Vol. 94 Issue 7, p1876 

    Studies the local eosinophil activity and activating cytokines in the inflamed lesions of colon and rectum in patients with ulcerative colitis and proctitis by researchers from Uppsala, Sweden. Efforts of the researchers to gain insight into the role of eosinophil granulocyte in bowel mucosa in...

  • Ulcerative Colitis.  // Ulcerative Colitis (National Digestive Diseases Info Clearinghou;2006, Vol. 06 Issue 1597, p1 

    The article provides information on ulcerative colitis. Ulcerative colitis is an inflammatory bowel disease (IBD) that causes ulcers in the lining of the rectum and colon and affects people of any age, specifically between aged 15 and 30. It enumerates its symptoms including anemia, fatigue and...

  • Variants of CARD15 are Associated with an Aggressive Clinical Course of Crohn's Disease–An IG-IBD Study. Annese, Vito; Lombardi, Giovanni; Perri, Francesco; D'Inc, Renata; Ardizzone, Sandro; Riegler, Gabriele; Giaccari, Stelio; Vecchi, Maurizio; Castiglione, Fabiana; Gionchetti, Paolo; Cocchiara, Elena; Vigneri, Sergio; Latiano, Anna; Palmieri, Orazio; Andriulli, Angelo // American Journal of Gastroenterology;Jan2005, Vol. 100 Issue 1, p84 

    BACKGROUND: Three major variants of the CARD15 gene confer susceptibility to Crohn's disease (CD). Whether or not these variants correlate with specific clinical features of the disease is under evaluation.AIM: We investigated the possible association of CARD15 variants with specific clinical...

  • Antineutrophil Cytoplasmic Antibodies in Japanese Patients With Inflammatory Bowel Disease: Prevalence and Recognition of Putative Antigens. Sugi, Kazunori; Saitoh, Osamu; Matsuse, Ryoichi; Tabata, Kazue; Uchida, Kazuo; Kojima, Keishi; Nakagawa, Ken; Tanaka, Seigou; Teranishi, Tsutomu; Hirata, Ichiro; Katsu, Ken-ichi // American Journal of Gastroenterology;May1999, Vol. 94 Issue 5, p1304 

    OBJECTIVE: Our aim was to investigate the prevalence of antineutrophil cytoplasmic antibodies (ANCA) in Japanese patients with ulcerative colitis (UC) and Crohn's disease (CD), and the putative antigens recognized by perinuclear staining pattern ANCA (p-ANCA)-positive sera. METHODS: Sera from UC...

  • Neutrophil-related immunoinflammatory disturbance in steroid-overdosed ulcerative colitis patients. Yoshiyama, Shigeyuki; Miki, Chikao; Okita, Yoshiki; Araki, Toshimitsu; Uchida, Keiichi; Kusunoki, Masato // Journal of Gastroenterology;2008, Vol. 43 Issue 10, p789 

    There is some evidence that large preoperative doses of steroids are a causative factor for postoperative higher morbidity in ulcerative colitis (UC) patients. This study aimed to assess steroid-related changes in functional profiles of neutrophils in UC patients to estimate the immunological...

  • Dramatic And Long Lasting Efficiency (13 Years) Of Oral 5 Aspirin. Maroy, Bernard // Internet Journal of Academic Physician Assistants;2003, Vol. 3 Issue 1, p12 

    Introduction: Could 5 ASA be an option in case of cortico-resistant and/or intolerant ulcerative colitis? Case report: A 55-year-old woman was referred for a recent, moderately severe, left-sided ulcerative colitis. The response to corticotherapy was initially fair, then mediocre, complicated by...

  • The Microvacular Thrombi of Colonic Tissue in Ulcerative Colitis. Guobin He; Qin Ouyang; Daiyun Chen; Fengyuan Li; Jingguo Zhou // Digestive Diseases & Sciences;Sep2007, Vol. 52 Issue 9, p2236 

    Abstract  Mucosal microvacular thrombi in rectal biopsies were observed in some ulcerative colitis (UC). Heparin may be effective in steroid resistant UC in some studies, however, the new results of meta-analysis demonstrated a non-significant effect of heparin in controlled...

  • The Role of Zinc and Metallothionein in the Dextran Sulfate Sodium-Induced Colitis Mouse Model. C. Tran; S. Sundar; P. Coyle // Digestive Diseases & Sciences;Sep2007, Vol. 52 Issue 9, p2113 

    Abstract  Zinc (Zn) and its binding protein metallothionien (MT) have been proposed to suppress the disease activity in ulcerative colitis. To determine the role of Zn and MT in the dextran sulfate sodium (DSS)-induced model of colitis in mice, a DSS dose-response study was...


Read the Article


Sign out of this library

Other Topics