Anti-apoptosis Effects of Oxymatrine Protect the Liver from Warm Ischemia Reperfusion Injury in Rats

Hongchi Jiang; Fanqiang Meng; Jie Li; Xueying Sun
November 2005
World Journal of Surgery;Nov2005, Vol. 29 Issue 11, p1397
Academic Journal
Warm Ischemia and reperfusion (WI/R) results in the release of destructive proinflammatory cytokines and oxygen free radicals, which in turn cause injury to the liver. Apoptosis is regarded as the central mechanism of liver injury during WI/R. Oxymatrine, an extract from a traditional Chinese herb, Sophora flavescens Ait, has been widely used for the treatment of chronic hepatitis, by virtue of its anti-inflammatory and anti-apoptotic activity. The objective of this study was to investigate whether administration of oxymatrine could protect livers against WI/R. The experimental design consisted of three groups of rats (each group contained 10 Wistar rats): one group were treated by sham-operation; the second (control) group with WI/R were administrated saline, and the third group, rats with WI/R, were administered oxymatrine). Oxymatrine was intravenously administered before a 30-minute period of ischemia. Blood samples were collected for biochemical assay. Liver samples taken at different time points underwent histological examination for detection of apoptotic cells, and Western blotting analysis for Fas and Fas ligand, the key factors in the upper apoptotic pathways. Histologic alteration of the liver was attenuated in oxymatrine-treated rats, and the serum levels of AST and ALT were significantly (P « 0.01) reduced (73% and 61%, respectively). Oxymatrine significantly inhibited cell apoptosis, as examined by terminal deoxynucteotidyl transferase-mediated dUTP nick end-labeling (TUNEL), and it reduced the apoptotic index by 65% (P « 0.05%) as detected by flow cytometry. The anti-apoptotic activity of oxymatrine depends mainly on downregulation of Fas and Fas ligand. The results of this study indicate that oxymatrine may represent a potent drug to protect the liver against WI/R injury.


Related Articles

  • Propofol Protects against Ischemia/Reperfusion Injury Associated with Reduced Apoptosis in Rat Liver. Abdel-Wahab, Ali F.; Al-Harizy, Wahid M. // ISRN Anesthesiology;2013, p1 

    Propofol is an intravenous anesthetic, reported to have a protective effect against ischemia/reperfusion (I/R) injury in heart and brain, but no definite data are available concerning its effect in hepatic I/R. This work investigated the effect of propofol anesthesia on hepatic I/R injury using...

  • Human heat shock protein 27 exacerbates ischemia reperfusion injury in rats by reducing the number of T regulatory cells. SUNYI YE; CHENXI ZHANG; JIE ZHOU; JUN CHENG; ZHEN LV; LIN ZHOU; HAIYANG XIE; JIAN WU; SHUSEN ZHENG // Molecular Medicine Reports;2014, Vol. 9 Issue 5, p1998 

    Ischemia reperfusion injury (IRI) occurs in almost every liver surgery and is associated with the reduction of the liver blood flow. Ischemia impairs liver function and can even cause liver failure following surgery. The present study aimed to identify a new molecular target allowing the...

  • Inhibition of Endogenous Hedgehog Signaling Protects Against Acute Liver Injury After Ischemia Reperfusion. Pratap, Akshay; Panakanti, Ravikiran; Yang, Ningning; Eason, James D.; Mahato, Ram I. // Pharmaceutical Research;Nov2010, Vol. 27 Issue 11, p2492 

    Purpose: Although Hedgehog (Hh) signaling is required for endodermal commitment and hepatogenesis, the possibility that it regulates liver injury after ischemia reperfusion (I/R) has not been considered. Therefore, we determined the expression pattern of Hh signaling and its role in liver injury...

  • Reversal of Hemorrhagic Shock-Associated Hepatic Ischemia-Reperfusion Injury With N-acetylcysteine. Allan, Patrick F.; Bloom, Brian B.; Wanek, Sandra // Military Medicine;Mar2011, Vol. 176 Issue 3, p332 

    Background: The underpinning pathophysiology, prevalence, and clinical relevance particular to hemorrhagic shock-induced liver damage have been explored only recently. Importantly, several investigators have revealed that an aberrant periportal endothelial response, characterized by the early...

  • Role of Nitric Oxide in Liver Injury. Chen, Tracy; Zamora, Ruben; Zuckerbraun, Brian; Billiar, Timothy R. // Current Molecular Medicine;Sep2003, Vol. 3 Issue 6, p519 

    The complex role of nitric oxide (NO) in the liver can be explained by its patterns of regulation and unique biochemical properties. With a broad range of direct and indirect molecular targets, NO acts as an inhibitor or agonist of cell signaling events. In the liver, constitutively generated NO...

  • Glutamine Does Not Protect Against Hepatic Warm Ischemia/Reperfusion Injury in Rats Noh, Joonhwa; Behrends, Matthias; Choi, Soojinna; Bedolli, Melanie A.; Yardi, Jason; Hirose, Ryutaro; Niemann, Claus U. // Journal of Gastrointestinal Surgery;Feb2006, Vol. 10 Issue 2, p234 

    The administration of glutamine before experimental ischemia/reperfusion (I/R) has been shown to protect intestinal, pulmonary, and myocardial tissue by inducing heat shock proteins (HSP). However, it is not known whether glutamine is protective for all organs. We therefore tested whether...

  • Remote Renal Injury Following Partial Hepatic Ischemia/Reperfusion Injury in Rats. Behrends, Matthias; Hirose, Ryutaro; Yeon Ho Park; Tan, Vivian; Kim Dang; Fengyung Xu; Se Hun Park; Niemann, Claus U. // Journal of Gastrointestinal Surgery;Mar2008, Vol. 12 Issue 3, p490 

    Liver ischemia/reperfusion has been shown to result in injury of remote organs such as the heart and lungs. Whether or not acute liver injury also results in kidney injury has so far not been adequately addressed. In anesthetized Wistar rats, partial (70%) normothermic hepatic ischemia was...

  • Liver repopulation with bone marrow derived cells improves the metabolic disorder in the Gunn rat. Muraca, M.; Ferraresso, C.; Vilei, M. T.; Granato, A.; Quarta, M.; Cozzi, E.; Rugge, M.; Pauwelyn, K. A.; Caruso, M.; Avital, I.; Inderbitzin, D.; Demetriou, A. A.; Forbes, S. J.; Realdi, G. // Gut;Dec2007, Vol. 56 Issue 12, p17125 

    Background: Reversible ischaemia/reperfusion (I/R} liver injury has been used to induce engraftment and hepatic parenchymal differentiation of exogenous β2-microglubulin-/Thy1+ bone marrow derived cells. Aim: To test the ability of this method of hepatic parenchymal repopulation,...

  • Protective effects of apocynin and allopurinol on ischemia/reperfusion-induced liver injury in mice. Ping-Guo Liu; Song-Qing He; Yan-Hong Zhang; Jian Wu; Sijens, Paul E. // World Journal of Gastroenterology;5/14/2008, Vol. 14 Issue 18, p2832 

    AIM: To determine the effects of allopurinol, an inhibitor of xanthine oxidase, and apocynin, an inhibitor of NADPH oxidase, on oxidant stress and liver injury caused by hepatic ischemia/reperfusion (I/R) procedure in mice. METHODS: Mice were pretreated with a xanthine oxidase inhibitor,...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics