Biocomposites of covalently linked glucose oxidase on carbon nanotubes for glucose biosensor

Jian Li; Yue-Bo Wang; Jian-Ding Qiu; Da-cheng Sun; Xing-Hua Xia
November 2005
Analytical & Bioanalytical Chemistry;Nov2005, Vol. 383 Issue 6, p918
Academic Journal
The formation of covalently linked composites of multi�walled carbon nanotubes (MWCNT) and glucose oxidase (GOD) with high-function density for use as a biosensing interface is described. The reaction intermediates and the final product were characterized by using FT�IR spectroscopy, and the MWCNT-coated GOD nanocomposites were examined by atomic force microscopy (AFM) and transmission electron microscopy (TEM). Interestingly, it was found that the GOD�MWCNT composites are highly water soluble. Electrochemical characterization of the GOD�MWCNT composites that were modified on a glassy carbon electrode shows that the covalently linked GOD retains its bioactivity and can specifically catalyze the oxidation of glucose. The oxidation current shows a linear dependence on the glucose concentration in the solution in the range of 0.5�40 mM with a detection limit of 30 �M and a detection sensitivity of 11.3 �A/mMcm2. The present method may provide a way to synthesize MWCNT related composites with other biomolecules and for the construction of enzymatic reaction-based biofuel cells and biosensors.


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