TITLE

Fluorometric determination of thiol redox state

AUTHOR(S)
Patsoukis, Nikolaos; Georgiou, Christos
PUB. DATE
November 2005
SOURCE
Analytical & Bioanalytical Chemistry;Nov2005, Vol. 383 Issue 6, p923
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
This work presents a fluorometric thiol redox state (TRS) method based on the thiol-specific fluorescent probe monobromobimane. It determines the concentrations of non-protein (NP) thiols glutathione, cysteine and N-acetylcysteine, the protein (P) thiols, as well as the contribution of these components to symmetric and mixed disulfides (NPSSR, NPSSC, NPSSCAc, PSSR, PSSC, PSSCAc, PSSP). The method is very sensitive since it measures as low as 30 pmol -SH groups in samples with a minimum of 1�5 mg total protein, making possible the measurement of oxidative stress�related TRS components even in biological fluids such as cerebrospinal.
ACCESSION #
18813187

 

Related Articles

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics