TITLE

Microstructured layers of spherical biofunctional core-shell nanoparticles provide enlarged reactive surfaces for protein microarrays

AUTHOR(S)
Borchers, Kirsten; Weber, Achim; Brunner, Herwig; Tovar, Günter E. M.
PUB. DATE
November 2005
SOURCE
Analytical & Bioanalytical Chemistry;Nov2005, Vol. 383 Issue 5, p738
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Nanostructured core-shell particles with tailor-made affinity surfaces were used to generate microstructured affinity surfaces by microspotting the particles to form densely packed amorphous nanoparticle layers. These layers provided a large reactive surface for the specific binding of protein ligands from aqueous solution. Biofunctional core-shell particles were synthesized for this purpose that consisted of a silica core with a diameter of 100 nm and an organic shell a few nm thick. The nanoparticle core was prepared by sol-gel chemistry and the shell formed in suspension by organosilane chemistry. The shell provided amino groups or carbonyl groups at its outer surface for subsequent covalent immobilization of streptavidin, rabbit IgG antibodies or goat IgG antibodies. AlexaFluor 647®-conjugated and biotinylated cytochrome C and CyDye-labeled anti-rabbit IgG and anti-goat IgG were probed as model analytes. The core-shell nanoparticles were spotted using a pin-ring micro-arrayer onto microscope glass slides that were coated with a polycation monolayer by dip-coating prior to nanoparticle deposition. Amorphous particle layers of well-defined thicknesses in the range of 100 nm to 2 μm were obtained by printing aqueous particle suspensions containing 5–500 mg/mL (0.5–50 wt%) of silica particles. The specific affinity of the plotted nanoparticulate capture surface was demonstrated by binding Cy3-labeled donkey anti-rabbit IgG and Cy5-labeled mouse anti-goat IgG to immobilized rabbit IgG and goat IgG particles. The signal intensity per spot increased for any given analyte concentration when the amount of particles per spot was augmented. This was attributed to the increasing integration of receptor molecules per surface footprint, which shifted the binding equilibrium towards the formation of the receptor–ligand complex. Additionally, the locally-increased supply of receptor molecules at the nanoparticulate microchip surface resulted in a wide dynamic range of 4 fM–20 nM (covering six orders of magnitude).
ACCESSION #
18755163

 

Related Articles

  • Continuous strain bursts in crystalline and amorphous metals during plastic deformation by nanoindentation. Li, H.; Ngan, A. H. W.; Wang, M. G. // Journal of Materials Research;Nov2005, Vol. 20 Issue 11, p3072 

    Using depth-sensing indentation with sub-nanometer displacement resolution, the plastic deformation of a range of materials, including a metallic glass, amorphous selenium, Ni3Al, pure Nb, Al, Cu, and Zn metals, and an Al-Mg alloy, has been investigated at room temperature. In amorphous...

  • Controlled free-form fabrication of nanowires by dielectrophoretic dispension of colloids. Schirmer, Niklas C.; Schwamb, Timo; Burg, Brian R.; Hotz, Nico; Poulikakos, Dimos // Applied Physics Letters;7/20/2009, Vol. 95 Issue 3, p033111 

    The vertical growth of conductive gold nanowires in ambient atmosphere through on-demand dielectrophoretically guided deposition of nanoparticle-laden colloids is demonstrated. Accelerated by dielectrophoresis, rapidly evaporating colloidal droplets leave a capillary nozzle (diameter of 500 nm)...

  • Preparation and characterisation of ultrafine amorphous alloy particles. Hu, Z.; Fan, Y.; Chen, Y. // Applied Physics A: Materials Science & Processing;1999, Vol. 68 Issue 2, p225 

    Abstract. Chemical preparation, characterisation, and properties are briefly reviewed for transition metal-metalloid ultrafine amorphous alloy particles (UAAP). Special attention is paid to the new solid-state chemical reaction method and the new systems, i.e. P-bearing and B-and-P-bearing...

  • Correlation of Microstructure and Mechanical Properties of Tantalum-Continuous-Fiber-Reinforced Amorphous Matrix Composite Processed by Liquid Pressing. Lee, Kyuhong; Lee, Sang-Bok; Lee, Sang-Kwan; Lee, Sunghak // Metallurgical & Materials Transactions. Part A;Apr2009, Vol. 40 Issue 4, p828 

    A Zr-based amorphous alloy matrix composite reinforced with tantalum continuous fibers was processed without pores or misinfiltration by liquid pressing, and their microstructures and mechanical properties were investigated. About 60 vol pct of tantalum fibers were homogeneously distributed...

  • Synthesis and thermal stability of ball-milled and melt-quenched amorphous and nanostructured Al-Ni-Nd-Co alloys. Calin, M.; Grahl, H.; Adam, M.; Eckert, J.; Schultz, L. // Journal of Materials Science;Aug2004, Vol. 39 Issue 16/17, p5295 

    In this work the Al85Ni9Nd4Co2 alloy was used as a starting point for examining the possibility of forming bulk glassy Al-based materials by combining rapid quenching and ball milling techniques. Fine glassy powders were obtained by ball milling melt-spun amorphous ribbons using a severe...

  • Structural and Microstructural Evolution in BaTiO3 Ceramic Nanoparticles. Mandal, T. K. // ICFAI Journal of Science & Technology;Jun2008, Vol. 4 Issue 2, p21 

    The structure and microstructure of BaTiO3 nanoparticles synthesized by polymeric precursor method are correlated with the mean crystallite size and tetragonality ratio, c/a. Williamson-Hall integral breadth method is used for evaluation of microstructural quantities like, mean crystallite size...

  • Electrical bistability and charge transport behavior in Au nanoparticle/poly(N-vinylcarbazole) hybrid memory devices. Pei Ying Lai; Chen, J. S. // Applied Physics Letters;10/13/2008, Vol. 93 Issue 15, p153305 

    Polymer memory devices using poly(N-vinylcarbazole) (PVK) as the active layer, incorporated with various weight ratios (0, 0.083, and 0.2) of dodecanethiol capped gold nanoparticles (Au NPs), are investigated. Electrical bistability is demonstrated for all three PVK-based devices, regardless of...

  • Increase of thermal resistance between a nanostructure and a surface due to phonon multireflections. Volz, Sebastian; Chapuis, Pierre-Olivier // Journal of Applied Physics;Feb2008, Vol. 103 Issue 3, p034306 

    The thermal resistance between a nanostructure and a half-body is calculated in the framework of particle-phonons physics. The current models approximate the nanostructure as a thermal bath. We prove that the multireflections of heat carriers in the nanostructure significantly increase...

  • Co nanostructure arrays in patterned polymeric template. Rosa, W. O.; Jaafar, M.; Asenjo, A.; Vázquez, M. // Journal of Applied Physics;Apr2009, Vol. 105 Issue 7, p07C108 

    Nanostructured polymer (polymethyl methacrylate) has been fabricated replicating the hexagonal symmetry ordering of an anodized Al template. A magnetic material, in this case Co, is then sputtered onto the polymer surface giving rise to a nanostructured surface thin layer keeping the high...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics