TITLE

High-performance liquid chromatography–tandem mass spectrometry for identification of isoflavones and description of the biotransformation of kudzu root

AUTHOR(S)
Yan Zhang; Qing Xu; Xiaozhe Zhang; Jiping Chen; Xinmiao Liang; Kettrup, Antonius
PUB. DATE
November 2005
SOURCE
Analytical & Bioanalytical Chemistry;Nov2005, Vol. 383 Issue 5, p787
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
High-performance liquid chromatography–tandem mass spectrometry has been used to identify isoflavone aglycones and glycosides in kudzu root. Fourteen isoflavones were detected. Among these, six were identified by comparison with authentic standards. Tentative identifications of the other isoflavones are based on UV spectra, mass spectra of protonated and deprotonated molecules, and MS–MS data. Several are reported for the first time in kudzu root. The bioactivity and bioavailability of isoflavone aglycones are usually greater than those of their glycosides. To improve the bioavailability of kudzu root isoflavones, crude β-glycosidases prepared from microbes were used to hydrolyze the isoflavone glycosides. Several MS modes are combined not only to identify the isoflavones in kudzu root, but also to describe the biotransformation of kudzu root isoflavone glycosides. It is also proved that crude β-glycosidases have high selectivity toward the O-glycosides of isoflavones.
ACCESSION #
18755143

 

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