TITLE

Use of solid-phase extraction to enable enhanced detection of acyl homoserine lactones (AHLs) in environmental samples

AUTHOR(S)
Schupp, Peter J.; Charlton, Timothy S.; Taylor, Michael W.; Kjelleberg, Staffan; Steinberg, Peter D.
PUB. DATE
September 2005
SOURCE
Analytical & Bioanalytical Chemistry;Sep2005, Vol. 383 Issue 1, p132
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A challenge for understanding the role of bacterial cell–cell signalling in the environment is the detection of those signals, which are often present in low (nmol L−1) concentrations. We describe here a simple purification method, solid-phase extraction (SPE), for increasing the sensitivity of detection for one such group of signals, acyl homoserine lactones (AHLs), in environmental samples. Spiking of dried marine sponge tissue ( Stylinos sp.) with AHLs resulted in detection down to 0.01 ppm for 3-oxo-hexanoyl homoserine lactone (3-oxo C6-HSL) and 1 ppm for hexanoyl homoserine lactone (C6-HSL). Compared with liquid extraction methods use of SPE resulted in twofold and tenfold improvements in sensitivity, respectively.
ACCESSION #
18674367

 

Related Articles

  • Simple and Fast SPE-UPLC-MS-MS Method for an Allergy Tablet Mixture of Acids and Bases. Chambers, Erin; Diehl, Diane; Mazzeo, Jeff // LC-GC Europe;Dec2005 Supplement, Vol. 18, p12 

    The article describes a novel approach to selecting solid-phase extraction methodology based on two protocols and four mixed-mode Oasis sorbents. The components of the tablets used in the study are ibuprofen, pseudoephedrine and chlorpheniramine. The method achieved excellent recoveries for both...

  • Advanced Topics in Solid-Phase Extraction: Chemistries. Majors, Ronald E. // LC-GC North America;Jan2007, Vol. 25 Issue 1, p16 

    The article discusses several advances in solid-phase extraction (SPE). SPE has branched out into other areas by employing new formats, new chemistries via novel sorption and partition mechanisms, multidimensional techniques, and miniaturized systems. The approaches used for automation include...

  • A Novel, Mixed-Mode Weak Cation Exchange SPE Sorbent: Oasis® WCX. Ziling Lu; Diehl, Diane M.; Trammell, Brian; Brousmiche, Darryl; Woods, Ermie; O'Gara, John; Iraneta, Pamela C.; Karm, Sue; Walter, Tom; Yuehong Xu; Boissel, Cheryl; Mazzeo, Jeffrey R.; Gu, Weigiang; Beardsley, Maria; Yung-Fong Cheng // LC-GC North America;Jun2004 Supplement, Vol. 22, p57 

    Investigates the Oasis® weak cation exchange solid-phase extraction sorbent to selectively extract three types of bases from rat plasma samples. Sample preparation for strong bases and quarternary amines; Description of the retention mechanism; Improvement of retention for all types of basic...

  • Modern Techniques for the Extraction of Solid Materials -- An Update. Majors, Ronald E. // LC-GC North America;Jul2006, Vol. 24 Issue 7, p648 

    Traditional methods for the sample preparation of insoluble solid materials have represented one of ' the more time consuming and labor intensive efforts in analysis. In this installment of "Sample Prep Perspectives," Ron Majors examines modern sample preparation methods for solids that often...

  • Solid Phase Extraction Method for Selective Determination of Phthalate Esters in the Aquatic Environment. Fatoki, O. S.; Noma, A. // Water, Air & Soil Pollution;Oct2002, Vol. 140 Issue 1-4, p85 

    The use of solid phase extraction and capillary GLC provides the basis for the selective determination of phthalate ester plasticizers in rivers and marine water samples. Of the several solvent ratios (methanol in dichloromethane) used for selective elution of phthalate esters from the C18 solid...

  • Sample Prep Perspectives. Hanwen Wang; Wenmin Liu; Yafeng Guan; Majors, Ronald E. // LC-GC Asia Pacific;Jun2004, Vol. 7 Issue 2, p8 

    Presents information on the validation of an in-tube solid phase microextraction device (SPME) for the analysis of organic contaminants in water. Information on the function of SPME; Process of dynamic extraction technique; Comparison of classic SPME with on-line in-tube SPME system.

  • Minimizing SPE Elution Volumes. Sadjadi, S.; Gerhards, P. // LC-GC Europe;Mar2005 Supplement, Vol. 18, p52 

    This research investigates the efficiency of silica and polymeric-based solid phase extraction (SPE) with elution volumes minimized to 100 μL. Analytes spiked in bovine plasma were extracted with SPEC® C18 and Focus® SPE followed by LC-MS-MS analysis.

  • Analysis of Degree of Extraction of Solid-Phase Particles during Separation of Non-Newtonian Suspensions in a Cylindrical Direct-Flow Hydrocyclone with Forced Flotation. Yablonskii, V. O. // Chemical & Petroleum Engineering;Nov/Dec2003, Vol. 39 Issue 11/12, p697 

    The article presents an analysis of degree of extraction of solid-phase particles during separation of non-Newtonian suspensions in a cylindrical direct-flow hydrocyclone with forced floatation. Use of hydrocyclones in different production processes for the separation of heterogeneous systems...

  • On-Line and Off-Line Application of Micro-SPE (MEPS). Dawes, Peter; Dawes, Ern; Wynne, Paul // LC-GC Europe;Sep2007 Applications, Vol. 20, p32 

    The article focuses on the process of on-line and off-line application of micro-Solid-phase extraction (SPE) and micro-extraction packed sorbent (MEPS). Such process includes various methods that improves recovery, greater speciation, and minimized solvent and sample consumption. The MEPS refers...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics