Use of solid-phase extraction to enable enhanced detection of acyl homoserine lactones (AHLs) in environmental samples

Schupp, Peter J.; Charlton, Timothy S.; Taylor, Michael W.; Kjelleberg, Staffan; Steinberg, Peter D.
September 2005
Analytical & Bioanalytical Chemistry;Sep2005, Vol. 383 Issue 1, p132
Academic Journal
A challenge for understanding the role of bacterial cell–cell signalling in the environment is the detection of those signals, which are often present in low (nmol L−1) concentrations. We describe here a simple purification method, solid-phase extraction (SPE), for increasing the sensitivity of detection for one such group of signals, acyl homoserine lactones (AHLs), in environmental samples. Spiking of dried marine sponge tissue ( Stylinos sp.) with AHLs resulted in detection down to 0.01 ppm for 3-oxo-hexanoyl homoserine lactone (3-oxo C6-HSL) and 1 ppm for hexanoyl homoserine lactone (C6-HSL). Compared with liquid extraction methods use of SPE resulted in twofold and tenfold improvements in sensitivity, respectively.


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