Glutaraldehyde-modified electrode for nonlabeling voltammetric detection of p16 INK4A gene

Cunwang Ge; Wujian Miao; Meiju Ji; Ning Gu
October 2005
Analytical & Bioanalytical Chemistry;Oct2005, Vol. 383 Issue 4, p651
Academic Journal
A nonlabeling electrochemical detection method for analyzing the polymerase-chain-reaction-amplified sequence-specific p16 INK4A gene, in which the basis for the covalent immobilization of deoxyribonucleic acid (DNA) probe is described, has been developed. The self-assembly process was based on the covalent coupling of glutaraldehyde (GA) as an arm molecule onto an amino-functional surface. The p16 INK4A gene was used as the model target for the methylation detection of early cancer diagnosis. An amino-modified DNA probe was successfully assembled on the GA-coupling surface through the formation of Schiff base under potential control. The hybridization of amino-modified DNA probes with the target was investigated by means of electrochemical measurements, including cyclic voltammetry and square wave voltammetry. Furthermore, the functions of GA coupling for sequence-specific detection were compared with those obtained based on mercaptopropionic acid. Hybridization experiments indicated that the covalent coupling of GA was suitable for the immobilization of DNA probe and was sensitive to the electrochemical detection of single-base mismatches of label-free DNA targets in hybridization. Moreover, reported probe-modified surfaces exhibited excellent stability, and the hybridization reactions were found to be completely reversible and highly specific for recognition in subsequent hybridization processes. The strategy provided the potential for taking full advantage of existing modified electrode technologies and was verified in microarray technology, which could be applied as a useful and powerful tool in electrochemical biosensor and microarray technology.


Related Articles

  • Printable Electrochemical Biosensors: A Focus on Screen-Printed Electrodes and Their Application. Keiichiro Yamanaka; Vestergaard, Mun'delanji C.; Eiichi Tamiya // Sensors (14248220);Oct2016, Vol. 16 Issue 10, p1 

    In this review we present electrochemical biosensor developments, focusing on screen-printed electrodes (SPEs) and their applications. In particular, we discuss how SPEs enable simple integration, and the portability needed for on-field applications. First, we briefly discuss the general concept...

  • Comparison of the RNA-amplification based methods RT-PCR and NASBA for the detection of circulating tumour cells. Burchill, S.A.; Perebolte, L.; Johnston, C.; Top, B.; Selby, P. // British Journal of Cancer;1/7/2002, Vol. 86 Issue 1, p102 

    Increasingly, reverse transcriptase polymerase chain reaction (RT-PCR) is used to detect clinically significant tumour cells in blood or bone marrow. This may result in a redefinition of disease-free and clinical relapse. However, its clinical utility may be limited by lack of automation or...


    This study aims to develop an electrochemical method for the detection of fluoxetine (FXT) in the aqueous media using a commercial boron-doped diamond (BDD) electrode. The electrochemical behaviour and detection of FXT at BDD electrode were studied using cycling voltammetry (CV),...

  • Development of Electrochemical Sensor for Detection of Mercury by Exploiting His-Phe-His-Ala-His-Phe-Ala-Phe Modified Electrode. Daud, Nurulhaidah; Yusof, Nor Azah; Tan Wee Tee // International Journal of Electrochemical Science;Jul2011, Vol. 6, p2798 

    A sensitive voltammetric method for detection of mercury ions is described which is made by modifying a gold electrode with 3-mercaptopropionic acid followed by covalent attachment of the octapeptide His-Phe-His-Ala-His-Phe-Ala-Phe to the self-assembled monolayer using carbodiimide coupling. A...

  • Differences and Molecular Immunohistochemical Parameters in the Subtypes of Infiltrating Ductal Breast Cancer. Cristina Bertolo; David Guerrero; Alicia Cordoba; Manel Esteller; Santiago Ropero; Jose Martinez-PeƱuela; Jose Lera // American Journal of Clinical Pathology;Sep2008, Vol. 130 Issue 3, p414 

    Breast cancer is a heterogeneous disease, and patients are categorized into subtypes according to gene expression. We studied the associations among molecular, immunohistochemical, and clinicopathologic features and their distribution according to the subtypes luminal, HER2, basal, and...

  • A Modified Lynch Syndrome Screening Algorithm in Colon Cancer. Roth, Rachel M.; Hampel, Heather; Arnold, Christina A.; Yearsley, Martha M.; Marsh, William L.; Frankel, Wendy L. // American Journal of Clinical Pathology;2015, Vol. 143 Issue 3, p336 

    Objectives: Somatic BRAF mutation in colon cancer essentially excludes Lynch syndrome. We compared BRAF V600E immunohistochemistry (IHC) with BRAF mutation in core, biopsy, and whole-section slides to determine whether IHC is similar and to assess the cost-benefit of IHC. Methods: Resection...

  • Mutation Detection by Real-Time PCR: A Simple, Robust and Highly Selective Method. Morlan, John; Baker, Joffre; Sinicropi, Dominick // PLoS ONE;2009, Vol. 4 Issue 2, p1 

    Background: Molecular tests for diagnosis of disease, particularly cancer, are gaining increased acceptance by physicians and their patients for disease prognosis and selection of treatment options. Gene expression profiles and genetic mutations are key parameters used for the molecular...

  • A rapid and specific immunosensor for the detection of aflatoxigenic Aspergilli. Sun, Xiulan; Yan, Li; Tang, Yanjie; Zhang, Yinzhi // European Food Research & Technology;Jun2012, Vol. 234 Issue 6, p1013 

    In this research, a rapid immunosensor targeting aflatoxigenic Aspergilli was developed based on impedimetric electrochemical technology, involving the use of polyclonal antibody specific to extracellular antigen produced by Aspergillus parasiticus GIM 3.395. This sensor was fabricated by...

  • Genetics.  // Current Medical Literature: Dermatology;2006, Vol. 11 Issue 1, p26 

    The article presents an abstract of the study "Analysis of T-cell Receptor Gene Rearrangement for Predicting Clinical Outcome in Patients with Cutaneous T-cell Lymphoma a Comparison of Southern Blot and Polymerase Chain Reaction Methods," by T. Juarez, S. N. Isenhath, N. L. Polissar and others.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics