A comparative study of extraction apparatus in HPLC analysis of ochratoxin A in muscle

Guillamont, E. Moreno; Lino, C. M.; Baeta, M. L.; Pena, A. S.; Silveira, M. I. N.; Vinuesa, J. Ma�es
October 2005
Analytical & Bioanalytical Chemistry;Oct2005, Vol. 383 Issue 4, p570
Academic Journal
Ochratoxin A (OTA) is a secondary fungal metabolite produced by several moulds, mainly by Aspergillus ochraceus and by Penicillium verrucosum, that occurs in meat products. The aim of this work was to optimize an efficient extraction procedure for the determination of OTA in muscle tissue in order to assess its occurrence in meat samples. Three different apparatus, a Waring blender, a switching apparatus, and an ultrasonic processor, were evaluated to verify the efficiency of extraction. The analytical methods proposed involve the extraction with chloroform-orthophosphoric acid, cleanup through an immunoaffinity column, high-performance liquid chromatography/fluorescence detection for separation and identification of OTA, and confirmation with liquid chromatography/FD after methylation of OTA in muscle tissue. The limit of quantification of the proposed method was 0.04 �g kg-1. Recoveries of OTA, using switching apparatus, ranged from 90.3 to 103.2% for chicken muscle spiked at 2.4 and 0.48 �g kg-1, respectively, with a within-day relative standard deviation of 17 and 15.3%. The proposed method was applied to 38 chicken, swine, and turkey muscle samples and the presence of OTA was confirmed in five samples. Finally, the estimated daily intake of OTA in this study was between 23 pg kg-1 body weight per day for swine samples and 18 pg kg-1 body weight per day for turkey samples.


Related Articles

  • ASK TIP SHEET. Joseph, Nicole // Newsweek;12/5/2005, Vol. 146 Issue 23, p72 

    This article answers the question of what causes the color difference between a turkey or chicken's white and dark meat. The darker color of dark meat is caused by a deeply tinted, oxygen-storing protein called myoglobin in the bird's muscles. Since both chickens and turkeys rely on their leg...

  • Gobble! Gobble! Jarrow, Gail // Highlights;Nov2000, Vol. 55 Issue 11, p24 

    Provides information on turkeys and discusses how a hen protects her poults from predators.

  • Toxico-Pathological Effects of In Ovo Inoculation of Ochratoxin A (OTA) in Chick Embryos and Subsequently in Hatched Chicks. Zahoor-ul-Hassan; Khan, Muhammad Zargham; Saleemi, Muhammad Kashif; Khan, Ahrar; Javed, Ijaz; Bhatti, Sheraz Ahmed // Toxicologic Pathology;1/1/2012, Vol. 40 Issue 1, p33 

    This study was designed to investigate the toxico-pathological effects of in ovo inoculation of ochratoxin A (OTA) in chicken embryos and subsequently in the hatching chicks. Nine hundred fertile white leghorn (WL) layer breeder eggs were divided into eight groups (A–H). Group A was...

  • Protective Effect of Hypophae Rhamnoides Oil Against Ochratoxicosis in Chickens. Solcan, Carmen; Gogu, Mihaela; Solcan, Gheorghe // Bulletin of the University of Agricultural Sciences & Veterinary;2011, Vol. 68 Issue 1, p350 

    The aim of this study was evaluate the prophylactic of oil Hypophae rhamnoides, when included in a diet containing ochratoxins and fed to broiler chicks. The criteria of the evaluation included body weight gain, haematological profile and biochemistry, in addition to associated lesions in...

  • ERRATUM.  // Poultry Science;Sep2011, Vol. 90 Issue 9, p2122 

    A correction to the article "Color Values and Other Meat Quality Characteristics of Breast Muscles Collected From 3 Broiler Genetic Lines Slaughtered at 2 Ages," that was published in the previous issue of the journal is presented.

  • Porcine/Chicken or Human Nephropathy as the Result of Joint Mycotoxins Interaction. Stoev, Stoycho D.; Denev, Stefan A. // Toxins;Sep2013, Vol. 5 Issue 9, p1503 

    A survey was made of the literature concerning the occurrence and incidence of mycotoxic nephropathy in pigs and chicks in different countries. Various etiological factors contributing to the development of the disease were considered. The main nephrotoxic fungi as well as the specific...

  • Toxic effects of aflatoxin B and ochratoxin A, alone and in combination, on chicken embryos. Edrington, T.; Harvey, R.; Kubena, L. // Bulletin of Environmental Contamination & Toxicology;Mar1995, Vol. 54 Issue 3, p331 

    The article presents a study on the toxic effects of ochratoxin A (OA) and aflatoxin B1 (AFB) on the chicken embryos. The study utilizes the Chick Embryotoxicity Screening Test (CHEST) bioassay technique to determine the effects of administering OA and AFB. The study reveals that administering...

  • Effect of Ochratoxin A on Body Weight, Feed Intake and Feed Conversion in Broiler Chicken. Masilamani Sakthivelan, Sigamani; Sudhakar Rao, Ganne Venkata // Veterinary Medicine International;2010, p1 

    The effect of ochratoxin A (OA) on the body weight, feed intake, and feed conversion was investigated in broiler chicken fed dietary levels of OA at 0, 1, and 2 ppm for 28 days from hatch. Feeding OA significantly reduced the growth rate of broiler chicken. The reduction was observed from the...

  • Ochratoxin-A and Mold in Some Broiler Farms of Ismailia, Egypt and Evaluation of Common Mycotoxin Binders. Hassan, Ahmed M.; Youssef, Ahmed I.; Reddy, Pochana G. // International Journal of Poultry Science;2012, Vol. 11 Issue 4, p288 

    Ochratoxin A (OTA) is a serious problem affecting the poultry industry as well as a health hazard for humans consuming contaminated poultry meat. The aim of this study was to determine OTA levels in the feed used in some poultry farms in Ismailia, Egypt and in organs of birds from those farms....


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics