TITLE

Biosynthesis of Cd-bound phytochelatins by Phaeodactylum tricornutum and their speciation by size-exclusion chromatography and ion-pair chromatography coupled to ICP—MS

AUTHOR(S)
Loreti, Valeria; Toncelli, Daniel; Morelli, Elisabetta; Scarano, Gioacchino; Bettmer, Jörg
PUB. DATE
October 2005
SOURCE
Analytical & Bioanalytical Chemistry;Oct2005, Vol. 383 Issue 3, p398
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Cd-bound phytochelatins (Cd–PCs) have been synthesised by incubation of Phaeodactylum tricornutum cell cultures with Cd and purified by size-exclusion chromatography–UV–Vis. These complexes, which were identified in previous work, have now been used as model substances to develop and optimise ion-pair chromatography (IPC) coupled to inductively coupled plasma–mass spectrometry (ICP–MS) for analysis of Cd–PCs. Subsequent analysis of samples taken from Silene vulgaris plants cultivated under heavy metal stress conditions revealed Cd signals but no Cd–PC signals. By use of isotopically enriched 116Cd–PCs the sample preparation steps were verified to determine the stability of the analytes. We observed species transformation between Cd–PCs and other unidentified Cd complexes. Consequently, the kinetic and thermodynamic lability of Cd–PCs are decisive factors in their detection.
ACCESSION #
18591363

 

Related Articles

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics