Coexpression of CD40 and CD40 Ligand in Epstein-Barr Virus--Infected T and NK Cells and Their Role in Cell Survival

Imadome, Ken-Ichi; Shimizu, Norio; Arai, Ayako; Miura, Osamu; Watanabe, Ken; Nakamura, Hiroyuki; Nonoyama, Shigeaki; Yamamoto, Kohtaro; Fujiwara, Shigeyoshi
October 2005
Journal of Infectious Diseases;10/15/2005, Vol. 192 Issue 8, p1340
Academic Journal
We investigated the role that CD40-CD40 ligand (CD40L) signaling plays in survival of Epstein-Barr virus (EBV)-infected T and NK cells. EBV-infected T and NK cell lines derived from patients with either chronic active EBV infection (CAEBV) or nasal T/NK cell lymphoma, as well as virus-infected peripheral T cells freshly isolated from a patient with CAEBV, were shown to express both CD40 and CD40L on their surface. Apoptosis of these cells was enhanced by blockade of CD40-CD40L signaling by a fusion protein of CD40 and immunoglobulin G (CD40Ig). Expression of CD4O was induced in human CD40L-positive Jurkat T cells after experimental EBV infection, and apoptosis of infected cells was enhanced by CD40Ig. These results suggest that CD40-CD40L signaling promotes survival of EBV-infected T and NK cells and, thus, plays an important role in the pathogenesis of T/NK lymphoproliferative disorders associated with the virus.


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