Quantitative determination of Roundup Ready soybean ( Glycine max) extracted from highly processed flour

Corbisier, Philippe; Trapmann, Stefanie; Gancberg, David; Hannes, Liesbeth; Van Iwaarden, Pierre; Berben, Gilbert; Schimmel, Heinz; Emons, Hendrik
September 2005
Analytical & Bioanalytical Chemistry;Sep2005, Vol. 383 Issue 2, p282
Academic Journal
Roundup Ready soybean powder has been subjected to different amounts of DNA fragmentation to assess the accuracy of real-time PCR on processed food. Certified reference material (CRM) containing 10 g kg−1 of Roundup Ready soybean (ERM-BF410d) prepared by a dry-mixing processing method was exposed to water at two temperatures, using three different mixing devices, or to baking temperature (250°C) for 30 min. The amount of DNA extracted from the different samples was quantified by fluorimetry. The amount of fragmentation of the extracted DNA was characterised by gel and capillary electrophoresis and the percentage of genetically modified (GM) soybean was determined by a double quantitative real-time PCR method. Measurement of the event GTS 40-3-2 (RUR) was possible in all the treated materials, because small amplicons were amplified. Correct RUR percentages could be measured for intact powders with little or no DNA fragmentation. For samples with a high level of DNA degradation, however, the accuracy of the measurement was found to depend on the method used for DNA extraction. Genomic DNA isolated by use of silica resin resulted in statistically significant overestimation of the amount of GM.


Related Articles

  • The "Frankenfoods" Phenomenon. Farrell, Tony // Vegetarian Times;Mar2004, Issue 319, p79 

    Focuses on genetically modified soybeans. Processes of genetic modification (GM) in soybeans; Arguments of proponents of GM; Options for vegetarians.

  • A model to predict the frequency of integration of fitness-related QTLs from cultivated to wild soybean. Kitamoto, N.; Kaga, A.; Kuroda, Y.; Ohsawa, R. // Transgenic Research;Feb2012, Vol. 21 Issue 1, p131 

    With the proliferation of genetically modified (GM) products and the almost exponential growth of land use for GM crops, there is a growing need to develop quantitative approaches to estimating the risk of escape of transgenes into wild populations of crop relatives by natural hybridization. We...

  • Increase in GM crops challenges EU. Wright, Richard // Irish Farmers Monthly;Feb2013, p16 

    The article discusses the need for the European Union (EU) to secure genetically-modified (GM)-free soya following news that Brazil has increased its plantings of GM crops.

  • og WATCHDOG.  // Organic Gardening;Jul/Aug2003, Vol. 50 Issue 4, p10 

    Reports on the discovery of genetically engineered corn in Nebraska. Potential harmful effect of splicing pharmaceuticals into vegetables.

  • GM feed label proposal will raise import costs. Allison, Richard; Buss, Jessica // Farmers Weekly;2/8/2002, Vol. 136 Issue 6, p33 

    Focuses on the impact of European Union (EU) proposals for labelling genetically modified (GM) feed, on the costs of importing GM-free soya. Limit set for GM-free soyabean by the EU; Discussion on EU's aim to reinstate consumer confidence in food safety; Option to import soya from Brazil;...

  • August 2012 Crop Production: Corn, Soybean Production Down 13%, 12% from 2011.  // Corn & Soybean Digest Exclusive Insight;8/10/2012, p3 

    The article offers a production report for corn and soybean in the U.S. for August 2012. Corn production is projected at 10.8 billion bushels, down 13% from 2011 and the lowest production since 2006. As of August 1, 2012, yields are expected to average 123.4 bushels/acre, down 23.8 bushels from...

  • Options for Uneven Soybean Emergence, Stand.  // Corn & Soybean Digest Exclusive Insight;6/ 4/2013, p5 

    The article explores options for soybeans that are emerging and standing unevenly. Uneven soybean emergence linked to dry soil has reportedly prompted question about replanting. Two things to do before replanting due to an uneven stand are suggested which include digging around in areas where...

  • Co-Expression of Soybean Glycinins A1aB1b and A3B4 Enhances Their Accumulation Levels in Transgenic Rice Seed. Fumio Takaiwa; Chiyoko Sakuta; Seon-Kang Choi; Yoshifumi Tada; Takayasu Motoyama; Shigeru Utsumi // Plant & Cell Physiology;Oct2008, Vol. 49 Issue 10, p1589 

    The soybean major storage protein glycinin is encoded by five genes, which are divided into two subfamilies. Expression of A3B4 glycinin in transgenic rice seed reached about 1.5% of total seed protein, even if expressed under the control of strong endosperm-specific promoters. In contrast,...

  • A soybean (Glycine max) polyubiquitin promoter gives strong constitutive expression in transgenic soybean. Carlos Hernandez-Garcia; Robert Bouchard; John Finer // Plant Cell Reports;May2009, Vol. 28 Issue 5, p837 

    Abstract  The success of plant genetic transformation relies greatly on the strength and specificity of the promoters used to drive genes of interest. In this study, we analyzed gfp gene expression mediated by a polyubiquitin promoter (Gmubi) from soybean (Glycine max) in stably...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics