TITLE

A new method for C-terminal sequence analysis in the proteomic era

AUTHOR(S)
Samyn, Bart; Sergeant, Kjell; Castanheira, Pedro; Faro, Carlos; van Beeumen, Jozef
PUB. DATE
March 2005
SOURCE
Nature Methods;Mar2005, Vol. 2 Issue 3, p193
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The overall study of post-translational modifications (PTMs) of proteins is gaining strong interest. Beside phosphorylation and glycosylation, truncations of the nascent polypeptide chain at the amino or carboxy terminus are by far the most common types of PTMs in proteins. In contrast to the analysis of phosphorylation and glycosylation sites, relatively little attention has been paid to the development of approaches for the systematic analysis of proteolytic processing events. Here we present a new mass spectrometry (MS)-based strategy that allows the identification of the C-terminal sequence of proteins. The method can be directly applied to proteins cleaved with cyanogen bromide (CNBr) and purified either by SDS-PAGE, by two-dimensional (2D) PAGE or in solution, and it therefore eliminates the need for specific isolation of the C-terminal peptide. Using Shewanella oneidensis as a model system, we have demonstrated that this approach can be used for C-terminal sequence analysis at a proteomic scale. We also applied the method to study the C-terminal proteolytic processing of procardosin A.
ACCESSION #
18445988

 

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