Widespread Prevalence of Wolbachia in Laboratory Stocks and the Implications for Drosophila Research

Clark, Michael E.; Anderson, Cort L.; Cande, Jessica; Karr, Timothy L.
August 2005
Genetics;Aug2005, Vol. 170 Issue 4, p1667
Academic Journal
Wolbachia is an intracellular microbe harbored by a wide variety of arthropods (including Drosophila) and filarial nematodes. Employing several different strategies including male killing, induced parthenogenesis, cytoplasmic incompatibility, and feminization, and acting by as-yet-unknown mechanisms, Wolbachia alters host reproduction to increase its representation within a population. Wolbachia is closely associated with gametic incompatibility but also interacts with Drosophila in other, little understood ways. We report here significant and widespread infection of Wolbachia within laboratory stocks and its real and potential impact on Drosophila research. We describe the results of a survey indicating that ∼0% of stocks currently housed at the Bloomington Drosophila Stock Center are infected with Wolbachia. Cells of both reproductive tissues and numerous somatic organs harbor Wolbachia and display considerable variation in infection levels within and between both tissue types. These results are discussed from the perspective of Wolbachia's potential confounding effects on both host fitness and phenotypic analyses. In addition to this cautionary message, the infection status of stock centers may provide further opportunities to study the genetic basis of host/symbiosis.


Related Articles

  • Detection and characterization of Wolbachia infections in laboratory and natural populations of different species of tsetse flies (genus Glossina). Doudoumis, Vangelis; Tsiamis, George; Wamwiri, Florence; Brelsfoard, Corey; Alam, Uzma; Aksoy, Emre; Dalaperas, Stelios; Abd-Alla, Adly; Ouma, Johnson; Takac, Peter; Aksoy, Serap; Bourtzis, Kostas // BMC Microbiology;2012 Supplement, Vol. 12 Issue Suppl 1, p1 

    Background: Wolbachia is a genus of endosymbiotic a-Proteobacteria infecting a wide range of arthropods and filarial nematodes. Wolbachia is able to induce reproductive abnormalities such as cytoplasmic incompatibility (CI), thelytokous parthenogenesis, feminization and male killing, thus...

  • A single mutation results in diploid gamete formation and parthenogenesis in a Drosophila yemanuclein-alpha meiosis I defective mutant. Meyer, Régis E.; Delaage, Michèle; Rosset, Roland; Capri, Michèle; Aït-Ahmed, Ounissa // BMC Genetics;2010, Vol. 11, p104 

    Background: Sexual reproduction relies on two key events: formation of cells with a haploid genome (the gametes) and restoration of diploidy after fertilization. Therefore the underlying mechanisms must have been evolutionary linked and there is a need for evidence that could support such a...

  • Wolbachia-Induced Cytoplasmic Incompatibility Is Associated with Decreased Hira Expression in Male Drosophila. Ya Zheng; Pan-Pan Ren; Jia-Lin Wang; Yu-Feng Wang // PLoS ONE;2011, Vol. 6 Issue 4, p1 

    Background: Wolbachia are obligate endosymbiotic bacteria that infect numerous species of arthropods and nematodes. Wolbachia can induce several reproductive phenotypes in their insect hosts including feminization, male-killing, parthenogenesis and cytoplasmic incompatibility (CI). CI is the...

  • Rapid Sequential Spread of Two Wolbachia Variants in Drosophila simulans. Kriesner, Peter; Hoffmann, Ary A.; Lee, Siu F.; Turelli, Michael; Weeks, Andrew R. // PLoS Pathogens;Sep2013, Vol. 9 Issue 9, p1 

    The maternally inherited intracellular bacteria Wolbachia can manipulate host reproduction in various ways that foster frequency increases within and among host populations. Manipulations involving cytoplasmic incompatibility (CI), where matings between infected males and uninfected females...

  • Toward a Wolbachia Multilocus Sequence Typing System: Discrimination of Wolbachia Strains Present in Drosophila Species. Paraskevopoulos, Charalampos; Bordenstein, Seth R.; Wernegreen, Jennifer J.; Werren, John H.; Bourtzis, Kostas // Current Microbiology;Nov2006, Vol. 53 Issue 5, p388 

    Among the diverse maternally inherited symbionts in arthropods, Wolbachia are the most common and infect over 20% of all species. In a departure from traditional genotyping or phylogenetic methods relying on single Wolbachia genes, the present study represents an initial Multilocus Sequence...

  • Distribution and reproductive effectsof Wolbachia in stalk-eyed flies(Diptera: Diopsidae). WERREN ‡ // Heredity;Sep98, Vol. 81 Issue 3, p254 

    Wolbachia are cytoplasmically inherited bacteria capable of altering the reproductive biology of their hosts in a manner which increases their spread within a population. These microbes can cause cytoplasmic incompatibility, parthenogenesis and feminization of genetic males. Because Wolbachia...

  • Infectious parthenogenesis. Huigens, M.E.; Luck, R.F.; Klaassen, R.H.G.; Maas, M.F.P.M.; Timmermans, M.J.T.N.; Stouthamer, R. // Nature;5/11/2000, Vol. 405 Issue 6783, p178 

    Presents research that reports frequent horizontal transmission of Wolbachia bacteria from infected to uninfected wasp larvae sharing a common food source. Methods; Results; Conclusions.

  • Cardinium symbionts cause cytoplasmic incompatibility in spider mites. Gotoh, T.; Noda, H.; Ito, S. // Heredity;Jan2007, Vol. 98 Issue 1, p13 

    Intracellular symbiotic bacteria belonging to the Cytophaga–Flavobacterium–Bacteroides lineage have recently been described and are widely distributed in arthropod species. The newly discovered bacteria, named Cardinium sp, cause the expression of various reproductive alterations...

  • parthenogenesis.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p1716 

    A definition of the term "parthenogenesis" which refers to unisexual reproduction is presented.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics