TITLE

Treatment of ova bum in-induced experimental allergic bronchitis in rats by inhaled inhibitor of secretory phospholipase A2

AUTHOR(S)
Shoseyov, D.; Bibi, H.; Offer, S.; Schwob, O.; Krimsky, M.; Kleiman, M.; Yedgar, S.
PUB. DATE
September 2005
SOURCE
Thorax;Sep2005, Vol. 60 Issue 9, p747
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: The pathophysiology of asthma involves the action of inflammatory/allergic lipid mediators formed following membrane phospholipid hydrolysis by phospholipase A2 (PLA2). Cysteinyl leukotrienes are considered potent inducers of bronchoconstriction and airway remodelling. Ovalbumin (OVA) induced bronchoconstriction in rats is associated with increased secretory PLA2 (sPLA2) activation and cysteinyl leukotriene production, together with suppression of cytosolic PLA2 and prostaglandin E2. These processes are reversed when the animals are pretreated systemically with an extracellular cell impermeable sPLA2 inhibitor which also suppresses the early allergic reaction to OVA challenge. In this study we examine the capacity of the sPLA2 inhibitor to ameliorate inflammatory and allergic manifestations (early and late bronchoconstriction) of OVA induced allergic bronchitis in rats when the inhibitor was administered by inhalation to confine it to the airways. Methods: Rats sensitised with OVA were treated with the sPLA2 inhibitor hyaluronic acid-linked phosphatidyl ethanolamine (HyPE). The rats were divided into four groups (n = 10 per group): (1) naïve controls (no sensitisation/no treatment); (2) positive controls (sensitisation + challenge with OVA inhalation and subcutaneous injection of 1 ml saline before each challenge; (3) sensitisation + challenge with OVA and HyPE inhalation before every challenge; and (4) sensitisation + challenge with OVA and treatment with subcutaneous dexamethasone (300 μg) before each challenge as a conventional reference. Another group received no treatment with HyPE during the sensitisation process but only before or after challenge of already sensitised rats. Pulmonary function was assessed and changes in the histology of the airways, levels of cysteinyl leukotrienes in BAL fluid, and the production of nitric oxide (No) and tumour necrosis factor α (TNFα) by BAL macrophages were determined. Results: Inhalation of HyPE markedly suppressed OVA induced early and late asthmatic reactions as expressed by bronchoconstriction, airway remodelling (histology), cysteinyl leukotriene level in BAL fluid, and production of TNFα and NO by BAL macrophages. OVA induced bronchoconstriction in sensitised non-pretreated rats was also inhibited by inhalation of HyPE either before or after the challenge. Conclusions: These findings confirm the pivotal role of sPLA2 in the pathophysiology of both the immediate allergic response and the inflammatory asthmatic process. Control of airway sPLA2 may be a new therapeutic approach to the treatment of asthma.
ACCESSION #
18366930

 

Related Articles

  • Getting it off your chest. Greenhalgh, Trisha // Accountancy;Mar2004, Vol. 133 Issue 1327, p125 

    This article focuses on the prevalence of chronic bronchitis or chronic obstructive pulmonary disease (COPD) during the winter season. The defining characteristics of COPD are wheezing, recurrent flare-ups needing antibiotics, and shortness of breath. Cough is triggered by traffic fumes,...

  • The role of occupation in the development of chronic obstructive pulmonary disease (COPD). Meldrum, M.; Rawbone, R.; Curran, A. D.; Fishwick, D. // Occupational & Environmental Medicine;Apr2005, Vol. 62 Issue 4, p212 

    Discusses the role of occupation in the development of chronic obstructive pulmonary disease (COPD). Number of diagnosed COPD cases in England and Wales in 2005; Calculation of the population attributable risk for COPD and asthma by the American Thoracic Society; Workers associated with the...

  • Smoking habit, respiratory symptoms and lung function in young adults. Urrutia, Isabel; Capelastegui, Alberto; Quintana, José Maria; Muñiozguren, Nerea; Basagana, Xavier; Sunyer, Jordi // European Journal of Public Health;Apr2005, Vol. 15 Issue 2, p160 

    Background: Smoking is the primary cause of deterioration in forced expiratory volume (FEV1) and the development of chronic obstructive lung disease. This study assessed the independent association of smoking with respiratory symptoms and lung function in young adults who took part in the...

  • Anti-diabetic activity of ethanolic extract of Adhatoda zeylanica. Bhatt, Meenakshi; Juyal, Vijay; Singh, Anita // Drug Invention Today;May2010, Vol. 2 Issue 5, p247 

    Adhatoda zeylanica is indigenous to India, where it is found in sub-Himalayan track upto an altitude of 1000 m and in Maharashtra especially, in Kankan region. Besides India, it is found in Myanmar, Srilanka and Malaya.The leaves, flowers, fruits and roots are extensively used for treating cold,...

  • ASTHMA OR COPD?  // Breckenridge American;9/20/2008, Special section p10 

    The article discusses the similarities and differences between asthma and chronic obstructive pulmonary disease (COPD). Asthma is usually caused by allergens and irritants like pollen, animal dander and cold air, while COPD is usually caused by smoking. In asthma, the air passages to the lungs...

  • Acute bronchitis. Worrall, Graham // Canadian Family Physician;Feb2008, Vol. 54 Issue 2, p238 

    The article discusses on acute bronchitis. Acute bronchitis is defined as an acute infection of the tracheo-bronchial tree which usually manifests by productive coughing. Studies show that there is a considerable clinical overlap between acute bronchitis and other acute respiratory infections,...

  • The impact of care pathways for exacerbation of Chronic Obstructive Pulmonary Disease: rationale and design of a cluster randomized controlled trial. Vanhaecht, Kris; Sermeus, Walter; Peers, Jan; Lodewijckx, Cathy; Deneckere, Svin; Leigheb, Fabrizio; Decramer, Marc; Panella, Massimiliano // Trials;2010, Vol. 11, p111 

    Background: Hospital treatment of chronic obstructive pulmonary disease (COPD) frequently does not follow published evidences. This lack of adherence can contribute to the high morbidity, mortality and readmissions rates. The European Quality of Care Pathway (EQCP) study on acute exacerbations...

  • Sodium metabisulphite induced airways disease in the fishing and fish-processing industry. M. Steiner; A. Scaife; S. Semple; G. Hulks; J. G. Ayres // Occupational Medicine;Dec2008, Vol. 58 Issue 8, p545 

    Background Sodium metabisulphite (SMBS) is recognized as a potential cause of airway irritation and possibly occupational asthma, but awareness of its use in the fishing and fish-processing industry is low. Aims and methods To describe three cases of occupational airways disease due to SMBS...

  • Impulse oscillometry: an alternative modality to the conventional pulmonary function test to categorise obstructive pulmonary disorders. Al-Mutairi, S.; Sharma, P.; Al-Alawi, A.; Al-Deen, J. // Clinical & Experimental Medicine;Jun2007, Vol. 7 Issue 2, p56 

    Impulse oscillometry (IOS) was introduced as an alternative modality to the conventional pulmonary function test (cPFT) to test lung mechanics. The objective of this study is to assess the use of IOS as an alternative, or in conjunction with cPFT, to categorise an obstructive respiratory...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sign out of this library

Other Topics