TITLE

Chlamydia trachomatis Variant with Nonfusing Inclusions: Growth Dynamic and Host-Cell Transcriptional Response

AUTHOR(S)
Minsheng Xia; Suchland, Robert J.; Bumgarner, Roger E.; Tao Peng; Rockey, Daniel D.; Stamm, Walter E.
PUB. DATE
October 2005
SOURCE
Journal of Infectious Diseases;10/1/2005, Vol. 192 Issue 7, p1229
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We compared growth rate and host-cell transcriptional responses of a Chlamydia trachomatis variant strain and a prototype strain. Growth dynamics were estimated by 16S rRNA level and by inclusion-forming units (IFUs) at different times after infection in HeLa cells. When inoculated at the same multiplicity of infection and observed 24-48 h after infection, the variant 16S rRNA transcriptional level was 3%-4% that of the prototype, and the IFUs of the variant strain were 0.1%-1% those of the prototype. Specific host-cell transcriptional responses to the variant were identified in a global-expression microarray in which variant strain- infected cells were compared with mock-infected and prototype strain-infected cells. In variant strain-infected cells, 47% (16/34) of specifically induced host genes were related to immunity and 32% (8/25) of specifically suppressed genes were related to lipid metabolism. The variant strain grew significantly more slowly and induced a modified host-cell transcriptional response, compared with the prototype strain.
ACCESSION #
18331144

 

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