TITLE

Effect of culture conditions on morphological changes of Helicobacter pylori

AUTHOR(S)
Tominaga, Kazunari; Hamasaki, Norio; Watanabe, Toshio; Uchida, Toshiyuki; Fujiwara, Yasuhiro; Takaishi, Osamu; Higuch, Kazuhide; Arakawa, Tetsuo; Shii, Eiji; Kobayashi, Kenzo; Yano, Ikuya; Kuroki, Tetsuo
PUB. DATE
December 1999
SOURCE
Journal of Gastroenterology;1999 Supplement 11, Vol. 34, p28
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The morphological conversion of Helicobacter pylori from the spiral form lo the coccoid form may be the expression of a transitory adaptation to an unsuitable environment. The mechanism(s) of this conversion are not clear. In this study, we examined whether the morphological conversion of H. pylori is affected by various culture conditions, such as oxygen concentration. pH, temperature, or the presence of β-cyclodextrin. H. pylori (NTCC11916) was cultured on Brucella agar. followed by culture in Brucella broth containing 1% agar under several conditions. Morphological conversion of individual H. pylori on the agar plate was investigated with time after incubation under phase contrast microscopy. When H. pylori was inoculated in Brucella broth containing β-cyclodextrin, the spiral form of the organism was observed even after 6 days of incubation under standard culture conditions: 37°C. pH 7, and microaerobic atmosphere (5% O2/10% CO2/85% N2) (control). The morphological conversion of H. pylori was completed on day 3 in an aerobic atomosphere (20% O2 supply) and on day 2 in an undermicroaerobic atmosphere («0.1% O2). Its complete morphological conversion was observed at pH 8 on day 5 and at pH 4 on day 6. All of the H. pylori (100%) incubated at 20° or 42°C had converted from the bacillary to the coccoid form on day 4. Conditioned medium without β-cyclodextrin caused complete conversion on day 5. These results suggest that oxgen concentration, pH, temperature, and β-cyclodextrin may be related to the H. pylori morphological conversion from the bacillary to the coccoid form.
ACCESSION #
18317484

 

Related Articles

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics