Perfusion of an isolated jejunal loop in uremic rats: Effect of sodium deoxycholate

Ganeval, Dominique; Marche, Claudie; Drüeke, Tilman
January 1973
Kidney International;Jan1973, Vol. 3 Issue 1, p222
Academic Journal
The effect of sodium deoxycholate (NaDC) on net transintestinal movement of electrolyles, nitrogenous substances and water was studied in anesthetized uremic rats. A jejunal loop isolated in vivo was perfused for three hours using a closed circuit. When 2 mM NaDC was present in the lumen, a significant increase (P<0.001) in the net transfer of sodium, potassium and calcium from blood to lumen was observed when compared to control perfusion without NaDC. The net fluxes of urea, creatinine, uric acid and phosphorus from blood to lumen were expresssed in terms of intestinal clearances. Mean clearances were increased by a factor of 2.5 for urea, 6.8 for creatinine, 6.6 for uric acid and 9.7 for phosphorus. Net water flow which was directed from lumen to blood with control was near zero when 2 mM NaDC was added to the perfusate; the difference, however, was not statistically significant. The effect of NaDC on net solute transfer was reproducible in the same animal and it could be reversed by the replacement of NaDC solution with control perfusate. Gross and histological examination of the isolated loops revealed no significant alterations. The data show that NaDC increases jejunal permeability to nitrogenous substances and electrolytes in the blood-to-lumen direction along a concentration gradient.


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