TITLE

The distribution of p-aminohippuric acid in rat kidney slices. II. Depth of uptake

AUTHOR(S)
Wedeen, Richard P.; Weiner, Barry
PUB. DATE
January 1973
SOURCE
Kidney International;Jan1973, Vol. 3 Issue 1, p214
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Section freeze-dry autoradiography was used to measure the depth to which concentrative uptake of PAH-³H occurs within rat renal cortical slices in vitro under conditions of varied incubation time, temperature and PAH medium concentration. Depth of concentrative uptake appears to be proportional to incubation time and approximately proportional to the square root of PAH medium concentration. When the medium PAH concentration is 1.3 mg/100 ml, the slice-to-medium (S/M) concentration ratio increases progressively for three hours. However, autoradiography revealed that the PAH concentration within those tubules demonstrating cellular accumulation remained relatively constant after five minutes. Thus, the increase in the S/M ratio with time of incubation appears to reflect the increasing volume of tissue participating in concentrative transport. The maximum depth at which concentrative uptake occurs appears to be limited by oxygen availability. Autoradiography permits correction of tissue PAH concentration for the fraction of the slice actually showing cellular accumulation, and thus reduces error when slice uptakes under differing incubation conditions are compared.
ACCESSION #
18287649

 

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