The distribution of p-aminohippuric acid in rat kidney slices. I. Tubular localization

Wedeen, Richard P.; Weiner, Barry
January 1973
Kidney International;Jan1973, Vol. 3 Issue 1, p205
Academic Journal
Tubular localization of tritiated p-amino-hippuric acid (PAH) was determined by section freeze-dry auto-radiography in rat renal cortical slices in vitro. Autoradiographs were also prepared from (issue after incubation in tritiated alpha-aminoisobutyric acid (AIB), tritiated methoxy-inulin and 14C-sucrose. PAH and AIB were concentrated in the cells of proximal tubules. Inulin and sucrose were confined to the extra-cellular spaces. Neither AIB, inulin nor sucrose could be detected within the lumina of proximal tubules. Of the compounds studied, only PAH appeared in significant concentrations in the lumina of proximal tubules. PAH appeared to be secreted by uphill transport at both the antiluminal and luminal surfaces of proximal tubule cells. AIB appeared to be accumulated in proximal tubule cells by transport across the antiluminal membrane.


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