TITLE

Isolation and immunological characterization of human glomerular basement membrane antigens

AUTHOR(S)
Marquardt, Hans; Wilson, Curtis B.; Dixon, Frank J.
PUB. DATE
January 1973
SOURCE
Kidney International;Jan1973, Vol. 3 Issue 1, p57
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
About 5% of the cases of human glomerulonephritis are mediated by antiglomerular basement membrane (GBM) antibodies produced by the patient and capable of reacting with his own GBM antigen(s). To elucidate the nature of the antigen(s) in human antiGBM nephritis that reacts with antiGBM antibodies, insoluble human G8M was rendered soluble by digestion of the basement membrane collagen with collagenase or by extraction with 3.5 M K Br. Collagenase digestion and dialysis effectively solubilized over 98% of the GBM, while KBr extraction resulted in the solubilization of 23% of the dry membrane. The immunochemical characterization of the resultant GBM proteins with rabbit antiGBM antiserum absorbed with human plasma indicated the presence of at least seven distinct GBM specific antigens. Both collagenase-solubilized GBM and its KBr extract are antigenically reactive with renal eluates from patients with antiGBM nephritis as demonstrated by 1) absorption experiments, 2) double diffusion analysis anti 3) a radioimmune assay. Further purification of the nephritogenic antigen(s) has been achieved by affinity chromatography with specific rabbit antiGBM antibodies. To obtain such antibodies, rabbits were immunized with the immunoprecipitate formed between the KBr extract and antiGBM antibodies eluted from a nephritic kidney. The rabbit antisera were absorbed with human serum and then used in affinity chromatography to isolate the nephritogenic GBM antigens.
ACCESSION #
18287524

 

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