Isolation and immunological characterization of human glomerular basement membrane antigens

Marquardt, Hans; Wilson, Curtis B.; Dixon, Frank J.
January 1973
Kidney International;Jan1973, Vol. 3 Issue 1, p57
Academic Journal
About 5% of the cases of human glomerulonephritis are mediated by antiglomerular basement membrane (GBM) antibodies produced by the patient and capable of reacting with his own GBM antigen(s). To elucidate the nature of the antigen(s) in human antiGBM nephritis that reacts with antiGBM antibodies, insoluble human G8M was rendered soluble by digestion of the basement membrane collagen with collagenase or by extraction with 3.5 M K Br. Collagenase digestion and dialysis effectively solubilized over 98% of the GBM, while KBr extraction resulted in the solubilization of 23% of the dry membrane. The immunochemical characterization of the resultant GBM proteins with rabbit antiGBM antiserum absorbed with human plasma indicated the presence of at least seven distinct GBM specific antigens. Both collagenase-solubilized GBM and its KBr extract are antigenically reactive with renal eluates from patients with antiGBM nephritis as demonstrated by 1) absorption experiments, 2) double diffusion analysis anti 3) a radioimmune assay. Further purification of the nephritogenic antigen(s) has been achieved by affinity chromatography with specific rabbit antiGBM antibodies. To obtain such antibodies, rabbits were immunized with the immunoprecipitate formed between the KBr extract and antiGBM antibodies eluted from a nephritic kidney. The rabbit antisera were absorbed with human serum and then used in affinity chromatography to isolate the nephritogenic GBM antigens.


Related Articles

  • Mapping the Binding Sites of Anti-BP180 Immunoglobulin E Autoantibodies in Bullous Pemphigoid. Fairley, Janet A.; Chang Ling Fu; Giudice, George J. // Journal of Investigative Dermatology;Sep2005, Vol. 125 Issue 3, p467 

    Bullous pemphigoid (BP) is a subepidermal blistering disease characterized by autoantibodies against the hemidesmosomal protein BP180 (BPAg2, type XVII collagen). NC16A, a non-collagenous stretch of the BP180 ectodomain, is the primary target of pathogenic immunoglobulin (Ig)G autoantibodies and...

  • SOLUBLE CD59-ANTIGEN LEVELS ARE INCREASED IN RENAL DISEASE PLASMA. Vanderpuye, Oluseyi Adewale // American Journal of Biochemistry & Biotechnology;2013, Vol. 9 Issue 3, p329 

    Cluster of Differentiation antigen 59 (CD59) is a glycosyl phosphatidylinositol-linked protein that protects cells from damage by lytic terminal complexes of complement. Soluble forms of CD59 in serum have been little investigated in disease states. In the present study, by using a sandwich...

  • Formation and clearance of tubulointerstitial immune complexes in kidneys of rats immunized with heterologous antisera to Tamm-Horsfall protein. Friedman, Jeffrey; Hoyer, John R.; Seiler, Marcel W. // Kidney International;Apr1982, Vol. 21 Issue 4, p575 

    Tubulointerstitial immune complex nephritis was produced by passive immunization of rats with antisera to rat Tamm-Horsfall protein (TH), a surface membrane glycoprotein of the cells of the thick ascending limb of Henle's loop. Circulating anti-TH antibodies were deposited in the kidney after an...

  • Renal tubular antigen excretion in normal human urine. I. Immunochemical identification. Schoenfeld, Leon S.; Glassock, Richard J. // Kidney International;Jan1973, Vol. 3 Issue 1, p309 

    A tissue antigen which is derived from the cells of the renal tubular epithelium was isolated from normal human urine (URTE). The URTE antigen is immuno-logically identical with one of the membrane-bound proximal renal tubular antigens and has an electrophoretic mobility in the alpha-two...

  • Antigen Distribution in Mucocutaneous Biopsies of Human Paracoccidiomycosis. Sandoval, Marina; De Brito, T.; Sotto, Mirian N.; Santos, R. T.; Franco, M. F. // International Journal of Surgical Pathology;Jan1996, Vol. 3 Issue 3, p181 

    The authors studied the distribution of Paracocddioides brasiliensis antigen(s) in human skin and oral mucosa. In biopsies obtained from untreated patients showing the chronic form of the disease, the authors demonstrated the P. brasiliensis antigen using two polyclonal immune sera raised in...

  • Clinical significance of bone alkaline phosphatase isoforms, including the novel B1x isoform, in mild to moderate chronic kidney disease. Haarhaus, Mathias; Fernström, Anders; Magnusson, Martin; Magnusson, Per // Nephrology Dialysis Transplantation;Nov2009, Vol. 24 Issue 11, p3382 

    Background. Mineral bone disorder (MBD) is a common complication of chronic kidney disease (CKD) even during the early stages. Bone alkaline phosphatase (BALP) is a marker of bone formation and plays a pivotal role in the mineralization process. Three BALP isoforms (B/I, B1 and B2) have been...

  • Serum- and glucocorticoid-regulated kinase 1 is upregulated following unilateral ureteral obstruction causing epithelial-mesenchymal transition. Jizhong Cheng; Truong, Luan D.; Xiaoqian Wu; Kuhl, Dietmar; Lang, Florian; Jie Du // Kidney International;Oct2010, Vol. 78 Issue 7, p668 

    Obstructive nephropathy leads to chronic kidney disease, characterized by a progressive epithelial-to-mesenchymal cell transition (EMT)-driven interstitial fibrosis. To identify the mechanisms causing EMT, we used the mouse model of unilateral ureteral obstruction and found a rapid and...

  • Noninhibitory PAI-1 enhances plasmin-mediated matrix degradation both in vitro and in experimental nephritis. Huang, Y.; Border, W. A.; Lawrence, D. A.; Noble, N. A. // Kidney International;Aug2006, Vol. 70 Issue 3, p515 

    Plasminogen activator inhibitor-type 1 (PAI-1) is thought to be profibrotic by inhibiting plasmin generation, thereby decreasing turnover of pathological extracellular matrix (ECM). A mutant, noninhibitory PAI-1 (PAI-1R) was recently shown by us to increase glomerular plasmin generation and...

  • Multifactorial genesis of enhanced platelet aggregability in patients with nephrotic syndrome. Machleidt, C.; Mettang, T.; Stärz, E.; Weber, J.; Risler, T.; Kuhlmann, U. // Kidney International;Dec1989, Vol. 36 Issue 6, p1119 

    Platelet aggregation, β-thromboglobulin (β- TG) and platelet factor 4 (PF 4) release and thromboxane B2 (TxB2) formation in stimulated platelet-rich plasma were investigated in 13 patients with nephrotic syndrome who had normal serum creatinine levels (creatinine clearance > 70 ml/min/1.73...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics