Multiplex PCR on leptospiral isolates from Kolenchery, Kerala, India

Sugathan, S.; Varghese, T. P.
April 2005
Indian Journal of Medical Microbiology;Apr2005, Vol. 23 Issue 2, p114
Academic Journal
journal article
Human leptospirosis causes severe multiorgan dysfunctions that may end in multiorgan failure and death. The methods in hand for diagnosis of leptospirosis like culture, ELISA and MAT only help to confirm the disease, and are of little value in early detection. The aim of this study was to find out if the two sets of primers described earlier could detect all the isolates from the area, for the purpose of using the resultant database for early detection of leptospires in future from clinical specimens. The study was done on culture isolates from Jan 2000 to June 2002 attending the department of medicine, MOSC medical college hospital, Kolenchery, Kerala, India. DNA of 45 culture isolates were amplified by multiplex PCR using two sets of previously described primers, G1, G2 and B 64-I, B 64-II. Specific amplifications of either 285 or 563 bp size were obtained from all isolates included in the study indicating the utility of the multiplex PCR in the rapid detection of leptospires in clinical samples.


Related Articles

  • Expanding the Diagnostic Use of PCR in Leptospirosis: Improved Method for DNA Extraction from Blood Cultures. Villumsen, Steen; Pedersen, Rebecca; Krogfelt, Karen Angeliki; Jensen, Jørgen Skov // PLoS ONE;2010, Vol. 5 Issue 8, p1 

    Background: Leptospirosis is a neglected zoonosis of ubiquitous distribution. Symptoms are often non-specific and may range from flu-like symptoms to multi-organ failure. Diagnosis can only be made by specific diagnostic tests like serology and PCR. In non-endemic countries, leptospirosis is...

  • Potential differences between Leptospira serovars, host-adapted (Bratislava) and incidental (Copenhageni), in determining reproductive disorders in embryo transfer recipient mares in Brazil. Pinna, A.; Martins, G.; Hamond, C.; Medeiros, M. A.; de Souza, G. N.; Lilenbaum, W. // Veterinary Record: Journal of the British Veterinary Association;5/24/2014, Vol. 174 Issue 22, following p531 

    The objective was to investigate potential differences between two Leptospira serovars, host-adapted (Bratislava) and incidental (Copenhageni), in causing reproductive disorders in mares. From August 2009 to March 2011, 608 adult recipient mares from eight studs located in the state of Rio de...

  • Serology and PCR for bovine leptospirosis: herd and individual approaches. Otaka, D. Y.; Martins, G.; Hamond, C.; Penna, B.; Medeiros, M. A.; Lilenbaum, W. // Veterinary Record: Journal of the British Veterinary Association;3/31/2012, Vol. 170 Issue 13, p338 

    The article presents a study which compare the use of microscopic agglutination test (MAT) and polymerized chain reaction (PCR) in the diagnosis of leptospirosis in cattle. It states that 187 adult cows with reduced fertility from three herds in Rio de Janeiro, Brazil, were screened for...

  • INVESTIGAÇÃO DO CONHECIMENTO DE PESSOAS INFECTADAS POR Leptospira spp. E DA SOROPREVALÊNCIA, LEPTOSPIRÚRIA E LEPTOSPIREMIA DE SEUS CÃES CONTACTANTES. Gaspareto dos Santos, Leonardo; Trochmann Cordeiro, Carolina; da Costa Vieira, Rafael Felipe; Wischral Jayme Vieira, Thállitha Samih; Oliveira Stedile, Simone Tostes // Archives of Veterinary Science;2019, Vol. 24 Issue 4, p83 

    Leptospirosis is a zoonotic disease, endemic in Brazil, who affects several mammals, including man. Asymptomatic dogs can transmit and it is considered that the number of animals with this status is underestimated. Although most of studies being performed by serology, the serology doesn't...

  • Multivisceral organ failure related to leptospirosis in pregnant patient. Hicham, Sbai; Ihsane, Mellouki; Abderahim, El Bouazzaoui; Brahim, Boukatta; Labib, Smail; Mustapha, Harrandou; Mohamed, Khatouf; Adil, Ibrahimi; Abdelilah, Melhouf; Nabil, Kanjaa // Indian Journal of Critical Care Medicine;Jan/Feb2013, Vol. 17 Issue 1, p43 

    Leptospirosis is the most widespread zoonosis in the world. It is caused by pathogenic leptospira infection. This infection is also an uncommon cause of hepatorenal failure. Indeed, hemolysis, elevated liver enzyme levels and low platelet count syndrome, and acute fatty liver of pregnancy are...

  • Preimplantation genetic diagnosis for medium-chain acyl-CoA dehydrogenase (MCAD) deficiency. Sermon, K. // MHR: Basic Science of Reproductive Medicine;Dec2000, Vol. 6 Issue 12, p1165 

    Examines the use of polymerase chain reaction test at the single cell level for preimplantation genetic diagnosis (PGD) for medium-chain acyl-CoA dehydrogenase (MCAD) deficiency. Symptoms of MCAD deficiency on affected children; Amplification efficiency and allele drop-out rate of PGD test on...

  • Leptospirosis in a case of non-Hodgkin lymphoma. Shekatkar, Smita Benjamin; Harish, Belgode Narasimha; Parija, Subhash Chandra // Journal of Infection in Developing Countries;Dec2010, Vol. 4 Issue 12, p846 

    Leptospiral meningitis, which is an important feature of anicteric leptospirosis, is generally underdiagnosed. Serological tests are not very useful in diagnosis of leptospiral meningitis. Early detection by molecular tests such as PCR and prompt institution of therapy would be life-saving.

  • Serology and PCR for bovine leptospirosis: herd and individual approaches. Otaka, D. Y.; Martins, G.; Hamond, C.; Penna, B.; Medeiros, M. A.; Lilenbaum, W. // Veterinary Record: Journal of the British Veterinary Association;3/31/2012, Vol. 170 Issue 13, preceding p338 

    The article presents a study which investigates herd and individual approaches of serology and polymerase chain reaction (PCR) for bovine leptospirosis. The study conducted leptospirosis screening of three dairy herds with high levels of reproductive failure from Rio de Janeiro, Brazil....

  • Leptospirosis and an animal bite. Bedard, Brenden A.; Kennedy, Byron S.; Weimer, Anita C.; Petruso, Anthony; Magnussen, Richard // Annals of Tropical Medicine & Public Health;May/Jun2014, Vol. 7 Issue 3, p182 

    In October 2013, leptospirosis was identified in a 20-year-old male. The male was bitten on his hand by either his canine or a skunk while breaking up a fight between the two animals. Eight days after the bite, the male developed fever, headache, drowsiness, neck pain, nausea, vomiting,...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics