Three-dimensional confocal thermal imaging using anti-Stokes luminescence

Kachynski, A. V.; Kuzmin, A. N.; Pudavar, H. E.; Prasad, P. N.
July 2005
Applied Physics Letters;7/11/2005, Vol. 87 Issue 2, p023901
Academic Journal
High-resolution thermal images of dye-stained live cells are obtained using confocal anti-Stokes luminescence on a confocal laser scanning microscope. Stokes and anti-Stokes fluorescence signal ratio imaging, using dual laser excitation with a fast alternate line scan, is used to monitor dynamical temperature changes at submicrometer resolution. Temperature mapping of a dye-doped polymer film with local heating is shown as an example. Three-dimensional mapping of local temperature changes in living cells induced by an external laser as the heating source, is obtained with temperature resolution better than 0.5 K.


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