Electrical detection of immobilized proteins with ungated AlGaN/GaN high-electron-mobility Transistors

Kang, B. S.; Ren, F.; Wang, L.; Lofton, C.; Tan, Weihong W.; Pearton, S. J.; Dabiran, A.; Osinsky, A.; Chow, P. P.
July 2005
Applied Physics Letters;7/11/2005, Vol. 87 Issue 2, p023508
Academic Journal
Ungated AlGaN/GaN high-electron-mobility transistor (HEMT) structures were functionalized in the gate region with aminopropyl silane. This serves as a binding layer to the AlGaN surface for attachment of fluorescent biological probes. Fluorescence microscopy shows that the chemical treatment creates sites for specific absorption of probes. Biotin was then added to the functionalized surface to bind with high affinity to streptavidin proteins. The HEMT drain-source current showed a clear decrease of 4 μA as this protein was introduced to the surface, showing the promise of this all-electronic detection approach for biological sensing.


Related Articles

  • Integrated microfluidic probe station. Perrault, C. M.; Qasaimeh, M. A.; Brastaviceanu, T.; Anderson, K.; Kabakibo, Y.; Juncker, D. // Review of Scientific Instruments;Nov2010, Vol. 81 Issue 11, p115107 

    The microfluidic probe (MFP) consists of a flat, blunt tip with two apertures for the injection and reaspiration of a microjet into a solution-thus hydrodynamically confining the microjet-and is operated atop an inverted microscope that enables live imaging. By scanning across a surface, the...

  • Optical waveguides for the evanescent wave-induced cleavage of photolabile linker compounds. Schmitt, Katrin; Rist, Jonas; Hoffmann, Christian // Analytical & Bioanalytical Chemistry;Nov2011, Vol. 401 Issue 2, p777 

    Functional surfaces and especially the control of surface properties depending on external parameters such as light illumination have gained increasing importance in the last few years. We present the characterization of polymers from the cycloolefin (co)polymer class (COC/COP) functionalized...

  • Simulation of Field-Effect Biosensors (BioFETs) for Biotin-Streptavidin Complexes. Windbacher, Thomas; Sverdlov, Viktor; Selberherr, Siegfried; Heitzinger, Clemens; Mauser, Norbert; Ringhofer, Christian // AIP Conference Proceedings;1/4/2010, Vol. 1199 Issue 1, p507 

    Biologically sensitive field-effect transistors (BioFETs) are a promising technology for detecting pathogens, antigen-antibody complexes, and tumor markers. A BioFET is studied for a biotin-streptavidin complex. Biotin-streptavidin is used in detection and purification of various biomolecules....

  • Microfabricated mechanical biosensor with inherently differential readout. Savran, C.A.; Burg, T.P.; Fritz, J.; Manalis, S.R. // Applied Physics Letters;8/25/2003, Vol. 83 Issue 8, p1659 

    We report measurements with a micromachined mechanical biosensor that inherently suppresses background effects by producing a differential signal with respect to a reference. The sensor comprises two adjacent cantilevers with interdigitated fingers between them that allow interferometric...

  • Targeting of biotinylated compounds to its target tissue using a low-density lipoprotein receptor-avidin fusion protein. Lehtolainen, P.; Wirth, T.; Taskinen, A. K.; Lehenkari, P.; Leppänen, O.; Lappalainen, M.; Pulkkanen, K.; Marttila, A.; Marjomäki, V.; Airenne, K. J.; Horton, M.; Kulomaa, M. S.; Ylä-Herttuala, S. // Gene Therapy;Dec2003, Vol. 10 Issue 25, p2090 

    The very high binding affinity of avidin to biotin is one of the highest to occur in nature. We constructed a fusion protein composed of avidin and the endocytotic LDL receptor in order to target biotinylated molecules to cells of the desired tissues. In addition to the native avidin,...

  • Self-assembled nanotube field-effect transistors for label-free protein biosensors. Hu, P.; Fasoli, A.; Park, J.; Choi, Y.; Estrela, P.; Maeng, S. L.; Milne, W. I.; Ferrari, A. C. // Journal of Applied Physics;Oct2008, Vol. 104 Issue 7, p074310 

    A self-assembly method is developed to fabricate single-wall carbon nanotube field-effect transistors (SWNT-FETs). The electrode surface and the area between electrodes are modified with nonpolar groups (-CH3) and polar groups (-NH3+). SWNTs are selectively placed in the area between the...

  • Biophysics: Take the intracellular train. Helmer, Magdalena // Nature;9/11/2003, Vol. 425 Issue 6954, p140 

    Focuses on the study in which researcher Stefan Diez and colleagues have tapped the into the transport system to mechanically manipulate DNA molecules and explore the potential of the cell's molecular transport machinery for engineering complex nanostructures and nanocircuits. Role of the...

  • High-Contrast Fluorescence Microscopy for a Biomolecular Analysis Based on Polarization Techniques Using an Optical Interference Mirror Slide. Mitsuru Yasuda; Takuo Akimoto // Biosensors (2079-6374);2014, Vol. 4 Issue 4, p513 

    Fluorescence microscopy with an improved contrast for fluorescence images is developed using an optical interference mirror (OIM) slide, which can enhance the fluorescence from a fluorophore as a result of the double interference of the excitation light and emission light. To improve the...

  • Valor pronóstico de la asociación entre la expresión de maspin y la ocurrencia de apoptosis en tumores mamarios caninos. Pantiú, A. J.; Insfrán, R, M.; Merlo, W. A.; Ortega, H. H.; Rosciani, A. S. // Revista Veterinaria;2011, Vol. 22 Issue 2, p95 

    Maspin protein (mammary serine protease inhibitor) in humans is considered a tumoral suppressor with different mechanisms of action such as apoptosis. For this work we studied the association of occurrence between the presence of maspin protein and apoptosis in canine mammary carcinomas, as well...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics