TITLE

Gene Analysis of Steroid 5 α-Reductase 1 in Hyperandrogenic Women

AUTHOR(S)
Eminović, Izet; Komel, Radovan; Preżelj, Janez; Karamehić, Jasenko; Gavrankapetanović, Faris; Hejić, Bećir
PUB. DATE
August 2005
SOURCE
Croatian Medical Journal;2005, Vol. 46 Issue 4, p664
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Aim To examine the gene encoding for 5α reductase type 1 in hyperandrogenic women, and assess the association of its eventual mutations or polymorphisms with the development of the hyperandrogenic female pattern. Methods Sixteen hyperandrogenic women were included in the study. Single-stranded conformation polymorphism analysis (SSCP) and DNA sequencing were performed after polymerase chain reaction amplification of each of the 5 exons of the SRD5A1 gene in both hyperandrogenic and control group (16 participants). Results Sequence analysis identified the existence of many polymorphisms; in codon 24 of exon 1, GGC (Gly) into GAC (Asp); in codon 30 of exon 1, CGG (Arg) into CGC (Arg); in exon 3 codon 169, ACA to ACG (both encoding for threonine); in exon 5, AGA to AGG (both encoding for arginine, codon 260); and T/C polymorphism in intron 2. Polymorphisms were found in both groups. Conclusion Polymorphisms of SRD5A1 gene were the same in both hyperandrogenic and healthy women, indicating no significant associations of genetic polymorphisms/variations of SRD5A1 gene with clinical manifestations of hyperandrogenic disorders in women.
ACCESSION #
18007925

 

Related Articles

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics