TITLE

DNA Assays for Detection, Identification, and Individualization of Select Agent Microorganisms

AUTHOR(S)
Jones, Susan W.; Dobson, Michael E.; Francesconi, Stephen C.; Schoske, Richard; Crawford, Robert
PUB. DATE
August 2005
SOURCE
Croatian Medical Journal;2005, Vol. 46 Issue 4, p522
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The purpose of this article is to review the status of DNA assays used for the detection, identification, and individualization of Bacillus anthracis, Yersinia pestis, Francisella tularensis, Burkholderia mallei, and Brucella abortus. These select agent microorganisms are historically significant as they have either been used or experimented with as a bioweapon or as a terrorist agent and are the subject of intense research in the areas of biodefense and bioforensics. If the presence of a biological agent is suspected, sensitive and specific assays for rapid detection and identification are necessary. However, DNA methods for identification of the sample may also be applied in order to individualize the strain and potentially determine the source of the microorganism. Methods used at the Armed Forces Institute of Pathology (AFIP)for select agent microbial DNA analyses include DNA extraction, DNA quantitation, real-time polymerase chain reaction (real-time PCR) of genetic targets unique to the select agent microorganism, microbial 16S ribosomal RNA gene DNA sequencing, amplified fragment length polymorphism polymerase chain reaction (AFLP-PCR), and more recently, repetitive element polymerase chain reaction (REP-PCR)DNA finger-printing. The methodologies of 16S ribosomal RNA gene DNA sequencing and DNA fingerprinting of microorganisms are well established within the field of diagnostic microbiology for DNA identification purposes, as well as DNA typing for epidemiological and genetic relatedness studies. 16S ribosomal RNA gene DNA sequencing and AFLP DNA fingerprinting have been validated at the Armed Force Institute of Pathology (AFIP)laboratory for identification purposes and can be used as a possible strain typing tool for Bacillus anthracis Yersinia pestis Francisella tularensis aswellas Brucella and Burkholderia species. The continued development and implementation of new DNA based methods with increased sensitivity and defined specificity will be particularly useful for the detection of residual microbial DNA signature in situations where the microorganism has been rendered nonviable by decontamination procedures or not able to be cultured on microbiological media.
ACCESSION #
18007843

 

Related Articles

  • Simultaneous real-time PCR detection of Bacillus anthracis, Francisella tularensis and Yersinia pestis. Skottman, T.; Piiparinen, H.; Hyytiäinen, H.; Myllys, V.; Skurnik, M.; Nikkari, S. // European Journal of Clinical Microbiology & Infectious Diseases;Mar2007, Vol. 26 Issue 3, p207 

    This report describes the development of in-house real-time PCR assays using minor groove binding probes for simultaneous detection of the Bacillus anthracis pag and cap genes, the Francisella tularensis 23 KDa gene, as well as the Yersinia pestis pla gene. The sensitivities of these assays were...

  • The Potential of TaqMan Array Cards for Detection of Multiple Biological Agents by Real-Time PCR. Rachwal, Phillip A.; Rose, Helen L.; Cox, Victoria; Lukaszewski, Roman A.; Murch, Amber L.; Weller, Simon A. // PLoS ONE;Apr2012, Vol. 7 Issue 4, p1 

    The TaqMan Array Card architecture, normally used for gene expression studies, was evaluated for its potential to detect multiple bacterial agents by real-time PCR. Ten PCR assays targeting five biological agents (Bacillus anthracis, Burkholderia mallei, Burkholderia pseudomallei, Francisella...

  • The Potential of TaqMan Array Cards for Detection of Multiple Biological Agents by Real-Time PCR. Rachwal, Phillip A.; Rose, Helen L.; Cox, Victoria; Lukaszewski, Roman A.; Murch, Amber L.; Weller, Simon A. // PLoS ONE;Apr2012, Vol. 7 Issue 4, p1 

    The TaqMan Array Card architecture, normally used for gene expression studies, was evaluated for its potential to detect multiple bacterial agents by real-time PCR. Ten PCR assays targeting five biological agents (Bacillus anthracis, Burkholderia mallei, Burkholderia pseudomallei, Francisella...

  • The Bad "Fab Four" Bacillus anthracis, Yersinia pestis, Francisella tularensis, Brucella species. Hinkel, Marty // Journal of Continuing Education Topics & Issues;Aug2005, Vol. 7 Issue 3, p118 

    Provides information on several bacteria included in the Select Agents List of the U.S. Centers for Disease Control and Prevention. Bacillus anthracis; Yersinia pestis; Francisella tularensis; Brucella species.

  • Simultaneous measurement of specific serum IgG responses to five select agents. Biagini, R. E.; Sammons, D. L.; Smith, J. P.; MacKenzie, B. A.; Striley, C. A. F.; Robertson, S. A.; Snawder, J. E.; Quinn, C. P. // Analytical & Bioanalytical Chemistry;Jun2005, Vol. 382 Issue 4, p1027 

    Select Agents are defined by CDC and the USDA Animal and Plant Health Inspection Service (APHIS) as biological agents or toxins deemed a threat to public, animal, or plant health, or to animal or plant products. They are classified on the basis of their ease of dissemination, mortality/morbidity...

  • Rapid Focused Sequencing: A Multiplexed Assay for Simultaneous Detection and Strain Typing of Bacillus anthracis, Francisella tularensis, and Yersinia pestis. Turingan, Rosemary S.; Thomann, Hans-Ulrich; Zolotova, Anna; Tan, Eugene; Selden, Richard F. // PLoS ONE;Feb2013, Vol. 8 Issue 2, p1 

    Background: The intentional release of Bacillus anthracis in the United States in 2001 has heightened concern about the use of pathogenic microorganisms in bioterrorism attacks. Many of the deadliest bacteria, including the Class A Select Agents Bacillus anthracis, Francisella tularensis, and...

  • Development and Comparison of Two Assay Formats for Parallel Detection of Four Biothreat Pathogens by Using Suspension Microarrays. Janse, Ingmar; Bok, Jasper M.; Hamidjaja, Raditijo A.; Hodemaekers, Hennie M.; Van Rotterdam, Bart J. // PLoS ONE;Feb2012, Vol. 7 Issue 2, p1 

    Microarrays provide a powerful analytical tool for the simultaneous detection of multiple pathogens. We developed diagnostic suspension microarrays for sensitive and specific detection of the biothreat pathogens Bacillus anthracis, Yersinia pestis, Francisella tularensis and Coxiella burnetii....

  • Development and Comparison of Two Assay Formats for Parallel Detection of Four Biothreat Pathogens by Using Suspension Microarrays. Janse, Ingmar; Bok, Jasper M.; Hamidjaja, Raditijo A.; Hodemaekers, Hennie M.; Van Rotterdam, Bart J. // PLoS ONE;Feb2012, Vol. 7 Issue 2, p1 

    Microarrays provide a powerful analytical tool for the simultaneous detection of multiple pathogens. We developed diagnostic suspension microarrays for sensitive and specific detection of the biothreat pathogens Bacillus anthracis, Yersinia pestis, Francisella tularensis and Coxiella burnetii....

  • Artificial plasmid engineered to simulate multiple biological threat agents. Carrera, Monica; Sagripanti, Jose-Luis // Applied Microbiology & Biotechnology;Jan2009, Vol. 81 Issue 6, p1129 

    The objective of this study was to develop a non-virulent simulant to replace several virulent organisms during the development of detection and identification methods for biological threat agents. We identified and selected specific genes to detect Yersinia pestis, Francisella tularensis,...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics