TITLE

Oxidative metabolism of the normal rat glomerulus

AUTHOR(S)
Gregoire, F.
PUB. DATE
January 1975
SOURCE
Kidney International;Jan1975, Vol. 7 Issue 1, p86
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Glomeruli from adult normal male Wistar rats were obtained by teasing a cortex slice with stainless steel needles. The enzyme content and the morphologic aspect of these glomeruli were assessed as a preliminary step to further metabolic studies, Robinson's medium appeared to be the most suitable medium. There was no loss of glutamic dehydrogenase, glucose-6-phosphate dehydrogenase or acid phosphatase. Lactate dehydrogenase was lost to about 50%. Electron microscopy showed morphologic signs of damage in the podocytes. The glomerular oxygen uptake was measured with the help of the Cartesian diver technique, using approximately 20 glomeruli per assay. The endogenous respiratory rate was linear for at least three hours. The mean dry wt of lyophilized glomeruli was determined for 13 rats for which the glomerular oxygen uptake had been measured, and these data showed a glomerular Qo2 of 4 μl/hr/mg of dry wt. The following substances were tested for their influence on the oxygen uptake: acetate, α-oxoglutarate, citrate, oxalacetate, glutamate, alanine, all 10 mM; succinate, 2.5, 5 and 10 mM; glucose, 5, 10 and 20 mM; fructose 10 and 20 mM; and palmitate, Citrate increases the O2 uptake/hr glomerulus by 30%; glucose, 20 mM, by 30%; and succinate, 2.5 mM by 50%, and 10 mM by 190%. In a Robinson's medium containing 35 mg of albumin/ml, the endogenous respiration is not different from that obtained in the inorganic medium but the oxygen uptake is increased 26% by glucose, 10 mM. From these data, it can be concluded that the oxygen uptake of the glomerulus is small. This fact explains its resistance to anoxia. The systematic investigation of possible substrates indicate that glucose, citrate and succinate may play a role in supporting this small oxidative metabolism.
ACCESSION #
17965670

 

Related Articles

  • Formation of Stable Submicron Protein Particles by Thin Film Freezing. Joshua Engstrom; Edwina Lai; Baltej Ludher; Bo Chen; Thomas Milner; Robert Williams; G. Kitto; Keith Johnston // Pharmaceutical Research;Jun2008, Vol. 25 Issue 6, p1334 

    Abstract Purpose  Highly stable, submicron lactate dehydrogenase (LDH) and lysozyme particles may be produced by thin film freezing (TFF) of aqueous solutions followed by lyophilization. Methods  The LDH activity was determined by measuring the decrease in absorbance of NADH over...

  • Protein concentration and enzyme activities of fresh and frozen-thawed Persian sturgeon, Acipenser persicus (Borodin, 1897) semen. Aramli, Mohammad Sadegh; Kalbassi, Mohammad Reza; Nazari, Rajab Mohammad // International Aquatic Research;Mar2014, Vol. 6 Issue 1, p1 

    The membrane integrity of fish spermatozoa can be compromised after exposure to a freeze-thaw cycle, resulting in leakage of proteins in the seminal fluid. In the present work, the total protein concentration (TP) and enzyme activities of acid phosphatase (AcP), lactate dehydrogenase (LDH),...

  • Effect of doxorubicin on the biochemical activities of the male reproductive system of white mice, Mus musculus. Sridevi, Thiagarajan // Indian Journal of Science & Technology;Dec2011, Vol. 4 Issue 12, p1715 

    Doxorubicin is one of the chemotherapeutic agents in treating cancer. The present study was undertaken to investigate whether doxorubicin had any adverse effect when administered at the recommended dose. For this purpose, the effect of Doxorubicin on LDH, glucokinase, alkaline phosphatase, acid...

  • Photoradiation of perfused placental tissue – a suitable in vitro model for photodynamic therapy? Schmolling, J.; Brunken, M.; Richter, O.; Ulrich, U.; Krebs, D.; Schmidt, S. // Archives of Gynecology & Obstetrics;Nov2001, Vol. 265 Issue 4, p199 

    Our aim was to evaluate the isolated placental lobule as a model to study the cytotoxic effects of photodynamic therapy (PDT) in vitro. Ten human placental lobules were dually perfused with a modified medium 199 for a 4-hour period. Photosan III was added to the fetal perfusate at a dose of 5...

  • Ginkgolides protect primary cortical neurons from potassium cyanide-induced hypoxic injury. Li Zhu; You Xu; Fang Du; Zhong Ming Qian // Experimental Brain Research;May2007, Vol. 179 Issue 4, p665 

    In this study, we investigated the effects of ginkgolides (Gins A, B, C and J), the main constituent of the non-flavone fraction of EGb 761, on hypoxic injury induced by potassium cyanide (KCN) in primary cortical neurons. The neurons were pretreated with or without ginkgolides for 24 h before...

  • Eliminating LDH tests in acute myocardial infarction. Apgar, Barbara // American Family Physician;2/15/1998, Vol. 57 Issue 4, p820 

    Presents a study by D.C. Randall and D.L. Jones to eliminate lactate dehydrogenase testing in acute myocardial infarction, originally from the journal `Archives Internal Medicine,' volume 157, 1997 issue. Details on the study; Methology used in the study; Findings of the study.

  • Recognition Site for the Side Chain of 2-Ketoacid Substrate in D-Lactate Dehydrogenase. Ishikura, Yoshirou; Tsuzuki, Shino; Takahashi, O.; Tokuda, Chizuka; Nakanishi, Rie; Shinoda, Takeshi; Taguchi, Hayao // Journal of Biochemistry;Dec2005, Vol. 138 Issue 6, p741 

    Replacement of Tyr52 with Val or Ala in Lactobacillus pentosus d-lactate dehydrogenase induced high activity and preference for large aliphatic 2-ketoacids and phenylpyruvate. On the other hand, replacements with Arg, Thr or Asp severely reduced the enzyme activity, and the Tyr52Arg enzyme, the...

  • Importance of glycolysis and oxidative phosphorylation in advanced melanoma. Ho, Jonhan; de Moura, Michelle Barbi; Lin, Yan; Vincent, Garret; Thome, Stephen; Duncan, Lyn M.; Hui-Min, Lin; Kirkwood, John M.; Becker, Dorothea; Van Houten, Bennett; Moschos, Stergios J. // Molecular Cancer;2012, Vol. 11 Issue 1, p76 

    Serum lactate dehydrogenase (LDH) is a prognostic factor for patients with stage IV melanoma. To gain insights into the biology underlying this prognostic factor, we analyzed total serum LDH, serum LDH isoenzymes, and serum lactate in up to 49 patients with metastatic melanoma. Our data...

  • TEMPORAL EFFECTS OF CARBOSULFAN ON TESTICULAR BIOCHEMICAL PARAMETERS AND ENZYME ACTIVITIES IN ALBINO MICE. RAGHAVENDR A. L. KSHEERASAGAR; BASAPP A. B. KALIWAL // International Journal of Pharma & Bio Sciences;Jan-Mar2013, Vol. 4 Issue 1, pB-173 

    Carbosulfan a N- methyl insecticide was administered orally with an effective dose of 48 mg/kg body weight per day to mice for 5, 10 20 and 30 days to examine the temporal effects. Mice were sacrificed on 31st day. Treatment with Carbosulfan for 20 and 30 days caused significant decrease in...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics