Culture of isolated renal tubules: A method of assessing viability of normal and damaged cells

Ash, Stephen R.; Cuppage, Francis E.; Hodes, Marion E.; Selkurt, Ewald E.
January 1975
Kidney International;Jan1975, Vol. 7 Issue 1, p55
Academic Journal
The article presents a study analyzing the differences in growth requirements or viability of normal and regenerating kidney tubule cells. Researchers removed normal and regenerating tubules from kidney slices and placed them in controlled tissue culture conditions. Fetal proximal tubules were to be cultured and compared to regenerating tubules. The conditions were chosen to give maximal cell visualization and to be most conducive to culture of epithelial cells. Time lapse photography was chosen as a criterion for viability of cells. Researchers indicated that an adaptation of regenerating cells to sub-optimal conditions influences outside the tubule such as hormones.


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