TITLE

Interstitial cells of Cajal are involved in the afferent limb of the rectoanal inhibitory reflex

AUTHOR(S)
de Lorijn, F.; de Jonge, W. J.; Wedel, T.; Vanderwinden, J. M.; Benninga, M. A.; Boeckxstaens, G. E.
PUB. DATE
August 2005
SOURCE
Gut;Aug2005, Vol. 54 Issue 8, p1107
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background and aims: Interstitial cells of Cajal (ICC) have been shown to be involved in nitrergic neurotransmission of the lower oesophageal sphincter and pylorus. Here we studied the role of ICC and nitric oxide (NO) in the inhibitory neurotransmission of the murine internal anal sphincter (IAS). Methods: The rectoanal inhibitory reflex, rectal compliance, and relaxation of the isolated IAS to electrical stimulation were measured in controls, KIT W/KITWv "mice, and neuronal NO synthase (nNOS) deficient mice. In addition, we evaluated the effect of blockade of nNOS using N-nitro-L-arginine methyl ester. Distribution of nNOS positive neurones and ICC in the IAS was assessed immunohistochemically. Results: KIT positive ICC were present in a dense network in the IAS of controls but not in KIT w/KITWv mice. Relaxation of IAS muscle strips induced by electrical stimulation was diminished in nNOS" mice but not in KIT W/KITWv mice. Blockade of NOS reduced the relaxation of IAS muscle strips in both mice. Relaxation of the IAS to rectal distension was significantly diminished in KITW/KITWv mice and nNOS deficient mice. In concert, in vivo blockade of NOS attenuated the relaxation of the IAS in controls. No significant difference in compliance was found. Conclusion: The inhibitory innervation of the IAS and the rectoanal inhibitory reflex are mediated by NO and the rectoanal inhibitory reflex requires an intact network of ICC in the IAS. Thus both loss of nitrergic innervation and deficiency of ICC lead to impaired anal relaxation and may play an important role in rectal evacuation disorders.
ACCESSION #
17814004

 

Related Articles

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sign out of this library

Other Topics