TITLE

A sensitive and specific HPLC-MS method for the determination of sophoridine, sophocarpine and matrine in rabbit plasma

AUTHOR(S)
Yong-jiang Wu; Jian-jun Chen; Yi-yu Cheng
PUB. DATE
August 2005
SOURCE
Analytical & Bioanalytical Chemistry;Aug2005, Vol. 382 Issue 7, p1595
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A sensitive and specific method was developed for the determination of sophoridine (SRI), sophocarpine (SC) and matrine (MT) in rabbit plasma by HPLC-MS. After an administration of Kuhuang by injection, blood samples were collected and extracted with methanol. The extract solutions were analysed by HPLC-MS method. The separation was performed on a ZORBAX Extend-C18 column using methanol/water/diethylamine (50:50:0.07, v/v/v) as mobile phase. The quinolizidine alkaloids were detected by using mass spectrometry in the SIM mode. There was a good linear relationship between peak area and concentration of analytes over the concentration range of 13.2–995.0 ng mL−1 for SRI, 7.0–530.0 ng mL−1 for SC and 8.8–655.0 ng mL−1 for MT, respectively. The absolute recovery of this method was more than 57% for SRI, 87% for SC and 91% for MT. The accuracy of assay was more than 90%. The limits of detection (LODs) were 6.8 ng mL−1 for SRI, 3.5 ng mL−1 for SC and 4.2 ng mL−1 for MT, respectively. The limits of quantitation (LOQs) were 13.2 ng mL−1 for SRI, 7.0 ng mL−1 for SC and 8.8 ng mL−1 for MT, respectively. The intra-day and inter-day coefficients of variation (RSDs) were less than 10.1, 6.3 and 5.8% for SRI, SC and MT, respectively. The developed method was applied to determine the concentration–time profiles of SRI, SC and MT in rabbit plasma after injection of Kuhuang.
ACCESSION #
17719873

 

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