Investigations into the electrooxidation of guanosine-5′-triphosphate at the pyrolytic graphite electrode

Goyal, Rajendra N.; Tyagi, Anuradha
August 2005
Analytical & Bioanalytical Chemistry;Aug2005, Vol. 382 Issue 7, p1683
Academic Journal
The electrochemical oxidation of guanosine-5′-triphosphate has been investigated in phosphate-containing electrolytes in the pH range 1.5–10.9 at a pyrolytic graphite electrode by cyclic sweep voltammetry, spectral studies, bulk electrolysis and related techniques. In this pH range, the oxidation occurred in a single well-defined peak (Ia). The peak potential of oxidation peaks ( Ep) was found to be dependent on pH, concentration and sweep rate. The kinetics of the UV-absorbing intermediates was followed spectrophotometrically and the decay of the intermediate occurred in a pseudo-first-order reaction. The first-order rate constants for the disappearance of the UV-absorbing intermediate have also been calculated. The products of the electrode reaction were characterized by HPLC and GC/MS. A tentative mechanism for the formation of the products has also been suggested.


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