Specific molecule localization in microchannel laminar flow and its application for non-immobilized-probe analysis

Yamashita, Kenichi; Ogura, Daisuke; Yamaguchi, Yoshiko; Miyazaki, Masaya; Briones, Maria Portia; Nakamura, Hiroyuki; Maeda, Hideaki
August 2005
Analytical & Bioanalytical Chemistry;Aug2005, Vol. 382 Issue 7, p1477
Academic Journal
Microfluidic systems enable superior control of fluidics. We have developed a novel size-separation method utilizing secondary flow within a microchannel. Using confocal fluorescence microscopy and computer simulation, we confirmed that separation occurred as a result of specific molecular localization in the curving part of the microchannel. Maximum separation efficiency was achieved by optimizing microchannel design and flow rate for individual separation targets. In addition, more effective separation was achieved by use of plural microchannel curves. This method was used for sequence-selective DNA sensing. Double-stranded DNA formed by hybridization between target DNA and a complementary probe had different elution profiles from those of the single-stranded non-complementary sequence. Moreover, the response depends on the length of the DNA molecules. This method does not require immobilization of either probe or target DNA, because all reactions occurred in the solution phase. Such features may reduce experimental error and the difference between data from different operators.


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