Modification of renal cortical subcellular membrane phospholipids induced by mercuric chloride

Morrison, Aubrey R.; Pascoe, Nina
February 1986
Kidney International;Feb1986, Vol. 29 Issue 2, p496
Academic Journal
Administration of mercuric chloride (HgCl2, 6 mg/kg) to rabbits produced renal failure, with changes in serum creatinine from 1.01 ± 1 in controls to 6.46 ± 0.91 mg/dl 24 hr after administration. Mitochondria isolated from HgCl2-treated rabbits exhibited alterations in acceptor control ratios, with reduction to 1.9 ± 0.2 from 3.9 ± 1.2 in controls. Ultrastructurally, the mitochondria showed swelling and loss of inner mitochondrial membranes. Total lipids from mitochondria of control and treated rabbits were obtained by modified Folch extraction and phospholipids analyzed by TLC. Brush border membranes and basolateral membranes were prepared from control and HgCl2-treated kidneys 2 and 24 hr after HgCl2 administration. At 24 hr mitochondria showed a 36% fall in phosphatidylcholine (PC), a 36% fall in phosphatidylethanolamine (PE), and a 27% fall in cardiolipin. Brush border showed a decrease in phosphatidylserine (PS) of 29% and in PE of 40%. The basolateral membranes showed a reduction only in PE of 35%. At 2 hr post HgCl2. early changes are confined to the BBM and consist of a reduction in PE in this membrane. This changes in membrane phospholipids may be important in the functional derangements that occur at the cellular level after HgCl2 administration.


Related Articles

  • Effects of reduced renal mass on tissue lipids and renal injury in hyperlipidemic rats. Kasiske, Bertram L.; O'Donnell, Michael P.; Cleary, Margot P.; Keane, William F.; Phillips, Fred // Kidney International;Jan1989, Vol. 35 Issue 1, p40 

    Increasing evidence from experimental models of chronic renal failure suggests that abnormalities in lipid metabolism may contribute to progressive renal injury. In the present study, hyperlipidemic obese, and normolipemic lean Zucker rats were subjected to unilateral nephrectomy or sham surgery...

  • DYSLIPIDIMIA; TO DETERMINE THE VARIOUS LIPID PROFILE PATTERN IN PATIENTS OF CHRONIC RENAL FAILURE. Lohano, Ashok Kumar; Bhatti, Irfan Ahmed; Iqbal, Azhar; Jilani, Salman Shah // Professional Medical Journal;2015, Vol. 22 Issue 7, p865 

    Objective: To determine the various lipid profile pattern of dyslipidimia in patients of chronic renal failure. Study design: Cross-sectional study. Place and duration of study: This study was conducted at Nephrology department of Isra University hospital Hyderabad and Medicine Department Civil...

  • Lipid alterations in LLC-PK1 cells exposed to mercuric chloride. Troyer, Dean A.; Kreisberg, Jeffrey I.; Venkatachalam, Manjeri A. // Kidney International;Feb1986, Vol. 29 Issue 2, p530 

    We have studied the effects of HgCl2 on the lipids of LLC-PK, (pig kidney) epithelial cells. Our results show that treatment of cells with HgCl2 caused a rapid accumulation of unesterified fatty acids (particularly arachidonic acid) and lysophospholipids. A 27-fold increase in unesterified...

  • Grade of chronic renal failure, and acute and long-term outcome after percutaneous coronary interventions. Reinecke, Holger; Trey, Torsten; Matzkies, Fritz; Fobker, Manfred; Breithardt, Günter; Schaefer, Roland M. // Kidney International;Feb2003, Vol. 63 Issue 2, p696 

    Grade of chronic renal failure, and acute and long-term outcome after percutaneous coronary interventions. Background. Patients with moderate chronic renal failure have recently been identified to suffer from a markedly higher mortality after percutaneous coronary intervention (PCI). We focused...

  • Abstracts Dutch Society of Nephrology Amsterdam, The Netherlands May 20, 1978.  // Kidney International;Jan1979, Vol. 15 Issue 1, p104 

    Presents abstracts of papers presented to the Dutch Society of Nephrology in Amsterdam, Netherlands, on May 20, 1978. Pathophysiology of mercuric chloride-induced acute renal failure in awake rats; Incidence of tubular acidosis in patients with chronic renal failure; Focal segmental glomerular...

  • Cockcroft-Gault Underestimates Creatinine Clearance in Black Patients.  // Kidney;Jan/Feb98, Vol. 7 Issue 1, p5 

    Investigates the relationship between race and creatinine clearance in chronic renal insufficiency. Levels of creatinine excretion in black and non-black patients; Influence of diabetes on creatinine excretion; Condition where race strongly affected creatinine clearance.

  • Increased tubular creatinine secretion by remnant nephrons—unexplained but informative? Ellam, Tim // NDT Plus;Apr2011, Vol. 4 Issue 2, p138 

    A letter to the editor is presented in which the benefits of increased contribution of tubular secretion to creatinine clearance in chronic kidney disease (CKD) are discussed.

  • Use cystatin C to detect kidney problems.  // Clinical Advisor;Mar2011, Vol. 14 Issue 3, p33 

    The article reports on the use of cystatin C blood measurements in detecting kidney diseases.

  • A Study of Parathyroid Hormone, Total Calcium, Creatinine and Urea in Chronic Kidney Disease. Parmar, Jigar A.; Sagar, Anamika R.; Kapadia, Shruti; Joshi, Anant; Chakrabarti, M. // Indian Journal of Public Health Research & Development;Oct-Dec2015, Vol. 6 Issue 4, p47 

    Backgound: Chronic kidney disease is a worldwide public health problem. It is the 12th cause of death and the 17th cause of disability, respectively. Secondary Hyperparathyroidism is seen in Chronic Kidney Disease. Objective: The present study was planned to assess changes in Serum PTH, Serum...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics