Early glycosilation products induce glomerular hyperfiltration in normal rats

Sabbatini, Massimo; Sansone, Gennaro; Uccello, Francesco; Giliberti, Antonella; Conte, Giuseppi; Andreucci, Vittorio E.
October 1992
Kidney International;Oct1992, Vol. 42 Issue 4, p875
Academic Journal
The effects of early glycosilation products (Amadori Products, AP) were investigated in male Munich-Wistar rats to establish whether AP play a role in the pathogenesis of glomerular hyperfiltration of early diabetic nephropathy. To mimic such a condition, normal rats were transfused with blood containing in vitro glycated serum (Group GLYC) to achieve plasma levels of Amadori products similar to those measured in rats with streptozotocin-induced diabetes. Rats transfused with normal blood were used as control (Group CON). Glomerular hemodynamics was evaluated in basal condition (B) and during acute hyperglycemia (HG). Blood transfusion did not alter basal hemodynamics. In Group CON, HG determined a rise of single nephron GFR (41.4 ± 3.2 vs. 32.1 ± 1.8 nl/min, P < 0.005), secondary to the increase of afferent effective filtration pressure (EFPa, +19% vs. B, P < 0.01). Rats of Group GLYC, in B, had values of SNGFR higher than those of Group CON B (46.8 ± 3 nl/min, P < 0.05, ANOVA). This increase was mediated by a significant reduction of glomerular afferent arteriole (Ra, -38% vs. Group CON B, P < 0.05), a rise in hydrostatic gradient pressure in glomerular capillaries (AP, +17%, P < 0.05) and of EFPa (+31%, P < 0.05). During HG, a further increase of SNGFR (58.3 ± 2.6, P < 0.005 vs. all groups) was detected in rats of Group GLYC, due to a significant rise in glomerular plasma flow (P < 0.05 vs. CON B and CON HG), in P (P < 0.05 vs. all the groups) and EFPa (P < 0.05 vs. all the other groups). In conclusion, this study demonstrates that glomerular hyperfiltration can be reproduced in normal rats by increasing the plasma concentration of Amadori Products to the values observed in diabetic rats. The pattern of modifications in glomerular hemodynamics resembles that commonly described in diabetic hyperfiltering rats.


Related Articles

  • Glomerular hemodynamic responses to pregnancy in rats with severe reduction of renal mass. Deng, Aihua; Baylis, Chris // Kidney International;Jul1995, Vol. 48 Issue 1, p39 

    These studies investigate glomerular hemodynamic responses to pregnancy in rats with 5/6th reduction of renal mass of four weeks duration. Both preglomerular find efferent arteriolar resistances (RA and RE) fell significantly at midterm although single nephron glomerular filtration rate (SNGFR)...

  • Estado actual de la implementación de las ecuaciones de estimación del filtrado glomerular en los laboratorios españoles. Gràcia-Garcia, Sílvia; Montañés-Bermúdez, Rosario; Morales-García, Luis J.; José Díez-de los Ríos, M.; Jiménez-García, Juan A.; Macías-Blanco, Carlos; Martínez-López, Rosalina; Ruiz-Altarejos, Joaquín; Ruiz-Martín, Guadalupe; Sanz-Hernández, Sonia; Ventura-Pedret, Salvador // Nefrologia;2012, Vol. 32 Issue 4, p508 

    Introduction: In 2006 the Spanish Society of Clinical Biochemistry and Molecular Pathology (SEQC) and the Spanish Society of Nephrology (S.E.N.) developed a consensus document in order to facilitate the diagnosis and monitoring of chronic kidney disease with the incorporation of equations for...

  • Renal hemodynamics and glomerular morphology in repetitively pregnant aging rats. Baylis, Christine; Rennke, Helmut G. // Kidney International;Aug1985, Vol. 28 Issue 2, p140 

    Measurements were made of renal and glomerular hemodynamics and glomerular morphology in repetitively pregnant and age-matched virgin female Munich-Wistar nits. Repetitive pregnancies and lactation provide a stimulus to chronic increases in glomerular filtration rate (GFR) and these rats were...

  • Dietary protein restriction and glomerular permselectivity in nephrotoxic serum nephritis. Neugarten, Joel; Kozin, Arthur; Gayner, Robert; Schacht, Robert G.; Baldwin, David S. // Kidney International;Jul1991, Vol. 40 Issue 1, p57 

    We have previously demonstrated that long-term dietary protein restriction ameliorates proteinuria and limits glomerular structural protein in rats with nephrotoxic serum nephritis. In the present study, we examined the influence of short-term dietary protein restriction on glomerular...

  • Glomerular hemodynamics in mercury-induced acute renal failure. Wolfert, Allen I.; Laveri, Leigh A.; Reilly, Katherine M.; Oken, Keith R.; Oken, Donald E. // Kidney International;Aug1987, Vol. 32 Issue 2, p246 

    As manifest by tubular collapse and the virtual absence of flow into the glomerulotubular junction (GTJ), filtration in most nephrons (SNGFR) of rats poisoned with 9 mg/kg body wt HgCl2 16 to 28 hours earlier was virtually absent. Arterial colloid osmotic pressure (COPA) and Bow- man's space...

  • Proteinuria and its relation to cardiovascular disease. Currie, Gemma; Delles, Christian // International Journal of Nephrology & Renovascular Disease;2014, Vol. 7, p13 

    Chronic kidney disease (CKD) and its associated morbidity pose a worldwide health problem. As well as risk of endstage renal disease requiring renal replacement therapy, cardiovascular disease is the leading cause of premature death among the CKD population. Proteinuria is a marker of renal...

  • Does nitric oxide, an inflammatory mediator of glomerular mesangial cells, have a role in diabetic nephropathy? Pfeilschifter, Josef // Kidney International Supplement;Sep1995, Issue 51, pS-50 

    Presents a study which sought to investigate the role of nitric oxide in diabetic nephropathy. Capacity of bacteria to generate nitric oxide by reduction of nitrite or by oxidation of ammonia; Identification of mesangial cell as a major determinant in the regulation of glomerular filtration...

  • Chronic kidney disease and the aging population. Tonelli, Marcello; Riella, Miguel C // Kidney International;Mar2014, Vol. 85 Issue 3, p487 

    The article presents author's views on the chronic kidney disease (CKD) in older people. He discusses several facts which includes CKD as a major risk multiplier in diseases patients with diabetes, hypertension and heart disease, glomerular filtration rate (eGFR) and celebration of the 9th World...

  • Chapter 2. Definition and classification of CKD.  // Clinical & Experimental Nephrology;Jun2009, Vol. 13 Issue 3, p196 

    The article presents information about chronic kidney disease (CKD) and its classification. An index of kidney function, glomerular filtration rate (GFR) is used for the classification of CKD stages. For the diagnosis of various stages of CKD, each stage is represented by eGFR, such as 15, 30,...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics