Identification of a target antigen in human anti-tubular basement membrane nephritis

Fliger, F. David; Wieslander, Jörgen; Brentjens, Jan R.; Andres, Giuseppe A.; Butkowski, Ralph J.
March 1987
Kidney International;Mar1987, Vol. 31 Issue 3, p800
Academic Journal
Sera from two patients with primary antitubular-basement-membrane-mediated tubulointerstitial nephritis, one a renal allograft recipient and the other with spontaneous antitubular-basement-membrane disease, were analyzed for the specificity of their autoantibodies. Both sera had circulating antibodies that reacted by ELISA with extracts of tubular basement membrane from several species, but failed to react significantly with extracts of glomerular basement membrane. Reactive antigen was solubilized with 6 M guanidine-HCl, 6 M urea, with reduction and alkylation, and with sodium dodecylsulfate. Digestion of the basement membrane with collagenase released relatively small quantities of antigen from the membrane, and trypsin and pepsin destroyed its antigenicity. The antigenic activity was characterized with respect to its size distribution by gel filtration and by immuno-overlay analysis of protein blots. Collectively, the results indicate that the major reactivity of both sera is directed towards a Mr 58,000 component that is unique to the tubular basement membrane. Minor reactivities toward high molecular weight components common to both glomerular and tubular basement membranes were detected by immuno-overlay analysis. This study identities an antigen that is involved in human anti-tubular-basement-membrane-mediated tubulointerstitial nephritis, and demonstrates an advantage of the use of denaturing extraction over proteolytic methods to prepare the antigen.


Related Articles

  • French Society of Nephrology.  // Kidney International;Sep1983, Vol. 24 Issue 3, p404 

    Presents abstracts of articles published in the journal "Kidney International." "Hemodialysis Biocompatibility: Evaluation of Dialysale and Membranes Respective Blood Interactions," by B. Branger, R. Oules, C. Granolleras, G. Deschodt, D. Treissede, S. Shaldon, and H. Mion; "In Vitro Studies of...

  • Diagnosis, treatment and follow-up of 25 patients with melamine-induced kidney stones complicated by acute obstructive renal failure in Beijing Children’s Hospital. Qiang Sun; Ying Shen; Ning Sun; Gui Ju Zhang; Zhi Chen; Jian Feng Fan; Li Qun Jia; Hong Zhan Xiao; Xu Ran Li; Puschner, Birgit // European Journal of Pediatrics;Apr2010, Vol. 169 Issue 4, p483 

    A total of 25 Chinese patients aged 6 to 36 months hospitalised at Beijing Children’s Hospital due to melamine-induced kidney stones complicated by acute obstructive renal failure in 2008 were included in a study in order to diagnose and treat these special cases more effectively. Feeding...

  • Receptors in proximal tubular epithelial cells for tubulointerstitial nephritis antigen. Yong Chen; Krishnamurti, Uma; Wayner, Elizabeth A.; Michael, Alfred F.; Charonis, Aristidis S. // Kidney International;Jan1996, Vol. 49 Issue 1, p153 

    Tubulointerstitial nephritis antigen (TIN-ag) is a novel basement membrane macromolecule that is involved in human anti-tubular-basement-membrane-mediated tubulointerstitial nephritis. The presence of antibodies to TIN-ag may result in an alteration of proximal tubule epithelial cell interaction...

  • Physicochemical characteristics of glomerular basement membrane antigens in urine. Huttunen, Niilo-Pekka; Turner, Malcolm W.; Barratt, T. Martin // Kidney International;Sep1978, Vol. 16 Issue 3, p322 

    Glomerular basement membrane (GBM) antigens in pooled normal urine and the pooled urine of infants with congenital nephrotic syndrome of Finnish type (CNF) have been characterized by gel filtration, ion-exchange chromatography, and immunodiffusion or immunoelectrophoretic analysis. Gel...

  • Back to basics: acute kidney injury III. Ellis, Peter // Journal of Renal Nursing;May2013, Vol. 5 Issue 3, p148 

    The article focuses on the management of acute kidney injury (AKI) cases classified as pre-renal, renal and post-renal, and discusses the causes of AKI. It presents an approach and a care plan in managing AKI which involved several factors including the patient's medical history, clinical...

  • Chronic-on-acute kidney injury. Palevsky, Paul M // Kidney International;Mar2012, Vol. 81 Issue 5, p430 

    Although older teaching suggested that patients who survived an episode of acute kidney injury (AKI) had a benign course, recent studies have demonstrated that AKI is strongly associated with increased risk for development of progressive chronic kidney disease (CKD) and longer-term mortality....

  • Vicarious contrast excretion by the gallbladder in contrast nephropathy. Umana, Idopise E.; Dwyer, Jamie P. // Kidney International;Feb2009, Vol. 75 Issue 4, p446 

    The article reports on the diagnosis given to a 69-year-old man who was evaluated for symptoms of heart disease. The patient has underwent laboratory examinations, which include urinalysis and imaging of the kidney for possible acute renal failure. According to the result of a non-contrasted...

  • Acute hemodialysis of infants weighing less than five kilograms. Sadowski, Robert H.; Harmon, William E.; Jabs, Kathy // Kidney International;Mar1994, Vol. 45 Issue 3, p903 

    The records of 33 infants weighing 5 kg or less who received acute hemodialysis treatment at Children's Hospital between 1980 and 1991 were reviewed. Dialysis was initiated to treat hyperammonemia (8), primary renal or renovascular disease (7), and acute renal failure (18). The infants weighed...

  • Acute renal failure following multiple wasp stings. Bhatta, Nisha; Singh, Rupa; Sharma, Sanjib; Sinnha, Arvind; Raja, Shiv // Pediatric Nephrology;Dec2005, Vol. 20 Issue 12, p1809 

    We report the cases of two patients who developed acute renal failure following multiple wasp stings. Both patients required dialysis and recovered within 4 weeks. The kidney biopsy from one patient showed acute tubular necrosis with interstitial nephritis. One patient had complete recovery of...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics