TITLE

Platelet-activating factor-induced loss of glomerular anionic charges

AUTHOR(S)
Camussi, Giovanni; Tetta, Ciro; Coda, Renato; Segoloni, Giuseppe Paolo; Vercellone, Antonio
PUB. DATE
January 1984
SOURCE
Kidney International;Jan1984, Vol. 25 Issue 1, p73
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The urinary protein excretion rate, the glomerular localization of cationic proteins (CP) derived from platelets and polymorphonuclear neutrophils (PMN) and the loss of fixed anionic charges were studied in rabbits infused with synthetic 1-0-octadecyl-2-acetyl-sn-glyceryl-3-phosphorylcholine [platelet activating factor (PAF), 1.5 μg/kg in 2 ml of saline containing 0.25% bovine serum albumin (BSA)]. The urinary protein excretion rate, unaffected by diphenhydramine, an antihistaminic agent, reached its maximum at 180 min and decreased 24 hr after PAF infusion. The localization of CP derived from platelets and PMN was investigated by immunofluorescence using specific antisera. Platelet-derived CP were detectable in glomeruli at 15 min and. particularly. at 180 min after PAF infusion. Cells positive for CP derived from PMN accumulated within 15 min in the glomerular capillaries and, later 1180 min), cytoplasmic depletion and localization in glomerular capillary walls occurred. CP deposits were associated with the loss of fixed anionic charges as detected by ruthenium red and colloidal iron staining. Rabbits infused with 1-0-octadecyl-sn-glyceryl-3-phosphorylcholine (lyso-PAF) or saline-BSA alone had none of the alterations described above. The development of proteinuria, the glomerular localization of platelet-and PMN-derived CP and the concomitant loss of fixed anionic charges suggested the possibility that, once CP were released in the circulation from PAF-stimulated platelets and PMN, they bound to, and neutralized, fixed anionic charges, resulting in enhanced glomerular permeability.
ACCESSION #
17666015

 

Related Articles

  • Platelet cationic proteins are present in glomeruli of lupus nephritis patients. Camussi, Giovanni; Tetta, Ciro; Mazzucco, Gianna; Monga, Guido; Roffinello, Caterina; Alberton, Maura; Dellabona, Paolo; Malavasi, Fabio; Vercellone, Antonio // Kidney International;Oct1986, Vol. 30 Issue 4, p555 

    Synthetic polycations have been shown to bind and neutralize glomerular polyanions (GPA), thereby increasing the permeability of the glomerular capillary wall (GCW). In the present study it is demonstrated that human platelet-derived cationic proteins (HuPlt CP), which are able to increase...

  • Glomerular platelet-activating factor levels and origin in experimental glomerulonephritis. Lianos, Elias A.; Zanglis, Anthony // Kidney International;Feb1990, Vol. 37 Issue 2, p736 

    The content of platelet activating factor (PAF) in glomeruli isolated from rats with nephrotoxic serum glomerulonephritis (NSGN) was quantified at various stages of the disease and the role of complement, platelets and neutrophils in mediating changes in glomerular PAF levels was evaluated. PAF...

  • Role of platelet activating factor in hemodialysis. Tetta, Ciro; David, Salvatore; Biancone, Luigi; Canino, Francesco; Cambi, Vincenzo; Camussi, Giovanni // Kidney International Supplement;Jan1993, Issue 39, pS-154 

    A complex array of inflammatory mediators are generated as a consequence of blood contact with hemodialysis (HD) membranes. Beside complement activation, other mediators are involved in cell activation, and are thought possibly to be responsible for early and long-term multiple changes in...

  • Lamifiban: A Viewpoint by David J. Moliterno. Moliterno, D.J. // Drugs;Feb1999, Vol. 57 Issue 2, p222 

    Comments on lamifiban, one of the most potent members of the small-molecule class of IIb/IIIa inhibitors developed for intravenous administration. Kinetics which make lamifiban's antiplatelet effect highly dependent on renal function; Antiplatelet effect of peptidomimetrics; Benefits from...

  • Correction: Moharam et al., Inhibitory Effects of Phylligenin and Quebrachitol Isolated from Mitrephora vulpina on Platelet Activating Factor Receptor Binding and Platelet Aggregation.  // Molecules;Mar2014, Vol. 19 Issue 3, p3848 

    A correction to the article "Inhibitory Effects of Phylligenin and Quebrachitol Isolated from Mitrephora vulpina on Platelet Activating Factor Receptor Binding and Platelet Aggregation" that was published in the previous issue is presented.

  • Mechanisms and kinetics for platelet and neutrophil localization in immune complex nephritis. Johnson, Richard J.; Alpers, Charles E.; Pruchno, Charles; Schulze, Matthias; Baker, Patricia J.; Pritzl, Pam; Couser, William G. // Kidney International;Nov1989, Vol. 36 Issue 5, p780 

    We have previously, reported that both neutrophils (PMNs) and platelets mediate proteinuria m a model of subendothelial immune complex (IC) nephritis (GN) in the rat. In order to understand the interaction of PMNs and platelets in this model, we quantitated the uptake of 111In-labelled platelet...

  • The Accuracy of Platelet Counting in Thrombocytopenic Blood Samples Distributed by the UK National External Quality Assessment Scheme for General Haematology. De la Salle, Barbara J.; McTaggart, Paul N.; Briggs, Carol; Harrison, Paul; Doré, Caroline J.; Longair, Ian; Machin, Samuel J.; Hyde, Keith // American Journal of Clinical Pathology;Jan2012, Vol. 137 Issue 1, p65 

    A knowledge of the limitations of automated platelet counting is essential for the effective care of thrombocytopenic patients and management of platelet stocks for transfusion. For this study, 29 external quality assessment specimen pools with platelet counts between 5 and 64 x 109/L were...

  • Evaluation of Sensory and in Vitro Cardio Protective Properties of Sardine (Sardina pilchardus): The Effect of Grilling and Brining. Nasopoulou, Constantina; Psani, Evangelia; Sioriki, Eleni; Demopoulos, Constantinos A.; Zabetakis, Ioannis // Food & Nutrition Sciences;Sep2013, Vol. 4 Issue 9, p940 

    The aim of this study was to investigate the effect of grilling and brining on the sensory properties, the fillet fatty acid composition and the cardio-protective activity of sardine (Sardina pilchardus), studying the in vitro activity against Platelet-Activating-Factor (PAF) induced platelet...

  • Chemical clues to anaphylaxis.  // Foods Matter (UK);Mar2008, p13 

    The article discusses a research published in "The New England Journal of Medicine" which compared blood samples from anaphylaxis patients to those from people without anaphylaxis. People with anaphylaxis have high blood levels of inflammatory chemical called platelet-activating factor (PAF)....

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics