Renal handling of lysozyme in the rat

Ottosen, Peter D.; Bode, Folkert; Madsen, Kirsten M.; Maunsbach, Arvid B.
March 1979
Kidney International;Mar1979, Vol. 15 Issue 3, p246
Academic Journal
The aim of the present study is to analyze the renal handling of lysozyme with special reference to whether or not a transtubular transport of intact lysozyme occurs. Thirty minutes after injection of 125I-lysozyme, 41.3 ± (SEM) 1.7% of the injected dose is accumulated in the kidneys where it is primarily located in the lysosomes, as demonstrated by differential centrifugation and electron microscope autoradiography. Thirty minutes after injection of 125I-lysozyme into one animal (donor), blood from this animal was cross-circulated to a second animal (acceptor) and the uptake of 125I-lysozyme in the acceptor's kidneys determined. The uptake of 125I-lysozyme in the acceptor kidneys provides a sensitive method of detecting minor changes in the 125I-lysozyme concentration in the donor's blood during different experimental situations. Comparison of experiments with normal donors and binephrectomized donors demonstrates that the kidney removes more 125I-lysozyme from plasma by glomerular filtration than it adds by a hypothetical transtubular transport. Comparison of experiments with binephrectomized donors and normal donors given a continuous infusion to balance glomerular removal of lysozyme demonstrates that no significant transtubular transport takes place. The present study shows: (1) lysozyme is taken up in kidney tubules by endocytosis and is catabolized in the lysosomes, (2) transtubular transport of intact protein, if it exists at all, is less than 9% of the lysozyme present in the tubules during the present experimental conditions.


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