Effect of calcium and hydrogen ion on the fibrinolytic activity of isolated renal glomeruli from rat

Sraer, J. D.; Blanc, E.; Delarue, F.; Kanfer, A.; Ardaillou, R.; Richet, G.
March 1979
Kidney International;Mar1979, Vol. 15 Issue 3, p238
Academic Journal
The fibrinolytic activity of glomeruli isolated from rat kidney has been measured with 125 I-fibrin-coated tubes. This technique allows the study of the two successive steps of the reaction: the release of the activator of plasminogen from the cells in the presence of isolated glomeruli, and the enzymatic process itself in the presence of the supernatant obtained after a 30-min incubation period with isolated glomeruli and centrifugation. Calcium in the range of 0.01 to 1.0 mM increased the rate of fibrinolysis and shortened the lag-time preceding this effect. The fibrinolytic activity was also measured in the presence of isolated glomeruli as a function of the plasminogen concentration. Calcium at 1 mM clearly increased the maximum velocity but did not change the concentration of plasminogen corresponding to 50% of the maximum. Supernatants were obtained after preincubation of isolated glomeruli with increasing doses of calcium. Addition of these supernatants to the 125I-fibrin-coated tubes produced a progressive increase in the rate of fibrinolysis, depending on the dose of calcium. When calcium was added to the supernatants only, after having discarded the glomeruli, there was no change or, above 0.5 mM calcium, a slight inhibition of the enzymatic reaction itself. The fibrinolytic activity measured in the presence of isolated glomeruli was maximum at a pH of 8. There was a clear-cut interaction in this condition between pH and calcium concentration. This interaction was not observed when the isolated glomeruli had been discarded. These results clearly demonstrate the major role of calcium in the release from glomeruli of an activator of plasminogen.


Related Articles

  • Rab3D and annexin A2 play a role in regulated secretion of vWF, but not tPA, from endothelial cells. Knop, Markus; Aareskjold, Elin; Bode, Günther; Gerke, Volker // EMBO Journal;8/4/2004, Vol. 23 Issue 15, p2982 

    von-Willebrand factor (vWF) and tissue-type plasminogen activator (tPA) are products of endothelial cells acutely released into the vasculature following cell activation. Both factors are secreted after intraendothelial Ca2+ mobilization, but exhibit opposing physiological effects with vWF...

  • fibrinolytic drugs. Peters, Michael // BMA A-Z Family Medical Encyclopedia;2004, p308 

    An encyclopedia entry for the term "fibrinolytic drugs" is presented. These are also known as thrombolytic drugs, which are applied to dissolve blood clots.

  • STUDIES ON PHARMACOLOGICAL ENHANCEMENT OF BLOOD FIBRINOLYTIC ACTIVITY. Gormsen, Johs.; Laursen, Benedicte // Angiology;Sep1970, Vol. 21 Issue 8, p546 

    The fibrinolytic activity was studied in forty eight patients before, during, and after treatment with clofibrate, metformin, tolazamide or testosterone. The fibrinolytic activity was estimated by assays of plasminogen, fibrinogen, euglobulin clot lysis time and the occurrence of...

  • Associations of Thrombophilia, Hypofibrinolysis, and Retinal Vein Occlusion. Glueck, Charles J.; Ping Wang; Bell, Howard; Rangaraj, Venkat; Goldenberg, Naila // Clinical & Applied Thrombosis/Hemostasis;Oct2005, Vol. 11 Issue 4, p375 

    We prospectively assessed whether thrombophilia and hypofibrinolysis, amplified by thrombophilic hormone replacement therapy (HRT), were associated with retinal vein occlusion (RVO). We studied 44 cases (18 men, 26 women), ≥ 3 months after RVO, 42 with central RVO, 2 with branch RVO, in...

  • Fibrinolytic markers and vasodilatory capacity following acute exercise among men of differing training status. Baynard, Tracy; Jacobs, Helena M.; Kessler, Craig M.; Kanaley, Jill A.; Fernhall, Bo // European Journal of Applied Physiology;Nov2007, Vol. 101 Issue 5, p595 

    We evaluated the effect of differing physical activity patterns on fibrinolysis and vasodilatory capacity using a cross-sectional design with 16 endurance-trained (ET) (mean ± SE) (28 ± 6 years), 14 resistance-trained (RT) (28 ± 7 years), and 10 untrained (UT) (26 ± 7 years) men....

  • Thrombolytic Therapy. Baker Jr., William F. // Clinical & Applied Thrombosis/Hemostasis;Oct2002, Vol. 8 Issue 4, p291 

    The therapeutic use of thrombolytic agents is the natural result of the increasing understanding of the pathophysiologic mechanisms underlying normal and deranged thrombosis and fibrinolysis. Plasminogen activators capable of increasing the production of plasmin exhibit considerable efficacy in...

  • Comparative vertebrate fibrinolysis. Tentoni, Juan; Polini, Nélida Nora; Casanave, Emma Beatriz // Comparative Clinical Pathology;Jun2010, Vol. 19 Issue 3, p225 

    Fibrinolysis is a feature in the phylogeny of vertebrates. This review analyzes the fibrinolytic mechanism in different species compared to healthy adult humans. Additionally, it introduces the armadillo Chaetophractus villosus (Mammalia, Xenarthra, Dasypodidae) whose lytic process remains quite...

  • Dihydropyridine calcium antagonists increase fibrinolytic activity: a systematic review. Vergouwen, Mervyn D. I.; Vermeulen, Marinus; De Haan, Rob J.; Levi, Marcel; Roos, Yvo BWEM // Journal of Cerebral Blood Flow & Metabolism;Jul2007, Vol. 27 Issue 7, p1293 

    Calcium antagonists have been shown to be superior over other antihypertensive drugs to prevent stroke. Because this cannot be fully attributed to blood pressure lowering effects, other mechanisms seem to play a role. Previously we found in patients with subarachnoid hemorrhage that nimodipine...

  • Hypercoagulability in Cushing’s syndrome: the role of specific haemostatic and fibrinolytic markers. Darko Kastelan; Tina Dusek; Ivana Kraljevic; Ozren Polasek; Zlatko Giljevic; Mirsala Solak; Silva Salek; Jozo Jelcic; Izet Aganovic; Mirko Korsic // Endocrine (1355008X);Aug2009, Vol. 36 Issue 1, p70 

    Abstract   Objective Hypercoagulability is a commonly described complication in patients with Cushing’s syndrome. Recent clinical studies have indicated various abnormalities of coagulation and fibrinolysis parameters which may be related to that phenomenon. The aim of this study...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics