Expression of transforming growth factor-β1 and β2 in rat glomeruli

MacKay, Karen; Kondaiah, Paturu; Danielpour, David; Austin III, Howard A.; Brown, Peter D.
December 1990
Kidney International;Dec1990, Vol. 38 Issue 6, p1095
Academic Journal
The transforming growth factors-β are potent modulators of cell growth and extracellular matrix metabolism in most types of cultured cells. The distribution and functions of TGF-β in vivo are less well known. We utilized several different techniques including northern blots, a CCI-64 cell growth inhibition assay, and sandwich enzyme-linked immunosorbent assays (SELISA) to examine the expression of TGF-β1 and TGF-β2 in rat glomeruli. High levels of TGF-β1 mRNA and protein were found in glomeruli (56 ± 22 ng TGF-βl/g tissue). These levels were several-fold higher than those present in whole kidney (10 ± 5 ng/g). TGF-β2 mRNA was present in glomeruli but was not detected in whole kidney. TGF-β2 concentrations by SELISA were 19 ± 8 ng TGF-β2/g in glomeruli and less than 5 ng/g in whole kidney. Since TGF-β has such marked effects on cell growth, we also examined whether alterations in TGF-β expression were associated with the renal hypertrophy which follows unilateral nephrectomy. Expression of TGE-β1 mRNA decreased in glomeruli following nephrectomy. However, this was not associated with a significant fall in glomerular TGF-β1 protein concentration. Whole kidney levels of TGF-β1 and its mRNA were unchanged following nephrectomy. Similar results were obtained for TGF-β2. Our data document the presence of high concentrations of TGF-β1 and β2 and their corresponding mRNAs in normal rat glomeruli. These results suggest that TGF-β may play important regulatory roles in the normal glomerulus.


Related Articles

  • Hypoxia Upregulates the Synthesis of TGF-β1 by Human Dermal Fibroblasts. Falanga, Vincent; Su Wen Qian, Vincent; Danielpour, David; Katz, Matthew H.; Roberts, Anita B.; Sporn, Michael B. // Journal of Investigative Dermatology;Oct91, Vol. 97 Issue 4, p634 

    In this report, we have investigated the secretion and synthesis of transforming growth factor-β1 (TGF--β1) by human dermal fibroblast cultures β1) response to hypoxia (2% oxygen), and have compared it to standard oxygen culture condition (15% oxygen at the cell surface). Sandwich...

  • 303. Prior observations that TGF-β[sub] is involved in glomerular disease are extended. Goumenos, D.S.; Tsakas, S.; Meguid, A. El Nahas // Kidney;May/Jun2003, Vol. 12 Issue 3, p105 

    Objective: To study the site of synthesis and expression profile of TGF-β[sub1] in the renal tissue of patients with heavy proteinuria and examine the relation of this expression with urinary excretion of TGF-β[sub1]. Methodology: Twenty-five patients with heavy proteinuria (8.4 &plusmin;...

  • Glomerular expression of transforming growth factor—β (TGF-β) isoforms in mice lacking CD2-associated protein. Woroniecki, Robert P.; Schiffer, Mario; Shaw, Andrey S.; Kaskel, Frederick J.; Bottinger, Erwin P. // Pediatric Nephrology;Mar2006, Vol. 21 Issue 3, p333 

    Mice lacking CD2-associated protein (CD2AP−/−) develop glomerular lesions resembling human focal segmental glomerulosclerosis (FSGS) between 3–4 weeks of age and die approximately 2 weeks later from massive proteinuria and renal failure. The mechanisms involved in the...

  • Transforming growth factor beta and platelets in allergic rhinitis and sinusitis. Rosas, Alejandro; Valencia, Martha P.; Sánchez, Manuel; Bautista, Maura; Rico, Guadalupe; Vega, Gloria B. // Revista Alergia de Mexico;2011, Vol. 58 Issue 2, p93 

    Objective: To determine the TGF-β concentration in plasma and platelets in patients with allergic rhinitis or rhinosinusitis. Methods: The study group included 36 adult females, 6 with clinical and laboratory diagnoses of allergic rhinitis, 18 with allergic rhinitis and sinusitis...

  • Changes in Transforming Growth Factor-β1 in Gingival Crevicular Fluid of Patients with Chronic Periodontitis Following Periodontal Flap Surgery. VIKRAM, VIGNESH; RAMAKRISHNAN, T.; ANILKUMAR, K.; AMBALAVANAN, N. // Journal of Clinical & Diagnostic Research;Feb2015, Vol. 9 Issue 2, p13 

    Introduction: The aim of the study was to assess changes in Transforming Growth Factor-β1 (TGF-β1) levels in gingival crevicular fluid (GCF) and examine correlation of TGF-β1 levels and periodontal parameters: probing pocket depth (PPD) and clinical attachment level (CAL) in sites with...

  • High glucose promotes TGF-β1 production by inducing FOS expression in human peritoneal mesothelial cells. Kokoroishi, Keiko; Nakashima, Ayumu; Doi, Shigehiro; Ueno, Toshinori; Doi, Toshiki; Yokoyama, Yukio; Honda, Kiyomasa; Kanawa, Masami; Kato, Yukio; Kohno, Nobuoki; Masaki, Takao // Clinical & Experimental Nephrology;Feb2016, Vol. 20 Issue 1, p30 

    Background: High glucose (HG) induces production of transforming growth factor-beta1 (TGF-β1), but the mechanism remains elusive. The aim of this study was to determine the gene(s) involved in HG-induced TGF-β1 production in human peritoneal mesothelial cells (HPMCs). Methods: Microarray...

  • Levels of TGF-β in serum and culture supernatants of CD4CD25 T cells from patients with non-segmental vitiligo. Tu, Cai-Xia; Jin, Wan-Wan; Lin, Mao; Wang, Zhao-Hui; Man, Mao-Qiang // Archives of Dermatological Research;Nov2011, Vol. 303 Issue 9, p685 

    Compelling evidences support an autoimmune basis of non-segmental vitiligo, and dysregulation of CD4CD25 regulatory T cell (Treg) is assumed to contribute to the pathogenesis of vitiligo. Serum levels of transforming growth factor-β (TGF-β), an important immunoregulatory cytokine produced...

  • Diagnostic Value of Homocysteine and Other Preeclampsia Markers: Relationship with Severity. Mansour, Amal; Harb, Hisham; Abdelhafeez, Mohamed // International Journal of Biological Chemistry;2011, Vol. 5 Issue 4, p227 

    Homocysteine and some related cytokines are involved in Preeclampsia (PE) pathogenesis but their role in progression of the disease is controversial, accordingly the aim of this study was to evaluate maternal serum Homocysteine levels and relate them to tumor necrosis factor-alpha (TNF-α) and...

  • Growth differentiation factor 15 can distinguish between hypertrophic cardiomyopathy and hypertensive hearts. Hanatani, Shinsuke; Izumiya, Yasuhiro; Takashio, Seiji; Kojima, Sunao; Yamamuro, Megumi; Araki, Satoshi; Rokutanda, Taku; Tsujita, Kenichi; Yamamoto, Eiichiro; Tanaka, Tomoko; Tayama, Shinji; Kaikita, Koichi; Hokimoto, Seiji; Sugiyama, Seigo; Ogawa, Hisao // Heart & Vessels;Mar2014, Vol. 29 Issue 2, p231 

    To distinguish hypertrophic cardiomyopathy (HCM) from hypertensive left ventricular hypertrophy (H-LVH) based on a morphological examination is often challenging. Growth differentiation factor 15 (GDF-15) is a novel diagnostic and prognostic biomarker for several cardiovascular diseases. In...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics