TITLE

Glomeruli synthesize nitrite in experimental nephrotoxic nephritis

AUTHOR(S)
Cattell, Victoria; Cook, Terence; Moncada, Salvador
PUB. DATE
December 1990
SOURCE
Kidney International;Dec1990, Vol. 38 Issue 6, p1056
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Activated macrophages synthesize nitric oxide (NO) from L-arginine. In culture, the major stable end product is nitrite (NO2-). Activated macrophages accumulate in glomeruli and are responsible for injury in experimental immune complex glomerulonephritis. We examined NO2- production by isolated glomeruli and urinary NO2- in accelerated nephrotoxic nephritis in the rat. Normal glomeruli did not produce NO2- spontaneously or when stimulated with lipopolysaccharide (LPS) (1 μg/ml) or A23187 (2 μg/ml). Cultured mesangial cells at first or seventh passage did not produce NO2- spontaneously or when stimulated. Nephritic glomeruli spontaneously produced NO2- at all times studied: this production was maximal at 24 hours after induction of glomerulonephritis (158.4 ± 8.4 nmol/48 hr/ml, N = 3). The production of NO2- was inhibited 75 to 100% by NG-monomethyl-L-arginine (L-NMMA), and this inhibition was reversed by L-arginine, indicating NO2- production from L-arginine via NO. The production of NO2- was increased by LPS (1 μg/ml) at 2, 7 and 21 days. NO2- was undetectable in normal rat urine: however, it was present in urine of rats with glomerulonephritis (Day 0 to 1: 8161 ± 2605 nmol/24 hr, N = 12). The production of NO in nephritic glomeruli may have implications for both the mechanism of glomerular injury and glomerular hemodynamics.
ACCESSION #
17656833

 

Related Articles

  • Initiation and evolution of interstitial leukocytic infiltration in experimental glomerulonephritis. Lan, Hui Y.; Paterson, David J.; Atkins, Robert C. // Kidney International;Sep1991, Vol. 40 Issue 3, p425 

    Initiation and evolution of interstitial leukocytic infiltration in experimental glomerulonephritis. Most forms of glomerulonephritis have a significant interstitial leukocytic infiltrate which is associated with disease progression. However, there is little data concerning the timing, initial...

  • The role of lymphocytes in the experimental progressive glomerulonephritis. Ikezumi, Yohei; Kanno, Katsue; Karasawa, Tamaki; Gi Dong Han; Ito, Yumi; Koike, Hiroko; Toyabe, Shinichi; Uchiyama, Makoto; Shimizu, Fujio; Kawachi, Hiroshi // Kidney International;Sep2004, Vol. 66 Issue 3, p1036 

    The role of lymphocytes in the experimental progressive glomerulonephritis. Background. Glomerular accmulation of leukocytes, including lymphocytes, is a common feature in most types of glomerulonephritis. However, the role of lymphocytes in progressive glomerulonephritis has not been...

  • Glomeruli synthesize nitrite in active Heymann nephritis; the source is infiltrating macrophages. Cattell, Victoria; Largen, Penelope; de Heer, Emile; Cook, Terence // Kidney International;Nov1991, Vol. 40 Issue 5, p847 

    Glomeruli synthesize nitrite (NO2Ä€) in experimental nephrotoxic nephritis, a model of glomerulonephritis where infiltrating macrophages are pathogenic. NO2Ä€ synthesis was studied in active Heymann nephritis (AHN), a model of membranous glomerulonephritis in which macrophages have not...

  • Induction of nitric oxide synthase in rat immune complex glomerulonephritis. Jansen, Albertine; Cook, Terence; Taylor, G. Michael; Larger, Penelope; Riveros-Moreno, Valentina; Moncada, Salvador; Cattell, Victoria // Kidney International;Apr1994, Vol. 45 Issue 4, p1215 

    Nitric oxide (NO) is a biological mediator which is synthesized from L-arginine by a family of nitric oxide synthases (NOS). Previously we have shown that NO is synthesized ex vivo by glomeruli obtained from animals with acute immune complex glomerulonephritis. We have now sought evidence for...

  • Co-regulated expression of glomerular 12/15-lipoxygenase and interleukin-4 mRNAs in rat nephrotoxic nephritis. Katoh, Tetsuo; Lakkis, Fadi G.; Makita, Naomasa; Badr, Kamal F. // Kidney International;Aug1994, Vol. 46 Issue 2, p341 

    Arachidonate 12- and 15-lipoxygenase (LO) products art generate in experimental glomerulonephritis. 15-S-HETE (a 15-LO product) and lipoxins (interaction products between 5-LO and either 12-LO or 15-LO) counteract the proinflammatory actions of leukotrienes. IL-4 has been shown to up-regulate...

  • Role of iron in the tubulo-interstitial injury in nephrotoxic serum nephritis. Alfrey, Allen C.; Froment, Daniel H.; Hammond, William S. // Kidney International;Nov1989, Vol. 36 Issue 5, p753 

    We studied the possibility that tubule fluid iron could be involved in the pathogenesis of the tubulo-interstitial injury associated with primary glomerular disease. Tubule fluid iron is determined by the magnitude of the glomerular leak for transferrin and the iron saturation of transferrin. To...

  • RENAL PARTICIPANTS The pathobiology of chronic allograft nephropathy: Immune-mediated damage and accelerated aging. Joosten, Simone A.; Van Kooten, Cees; Sijpkens, Yvo W. J.; De Fijter, Johan W.; Paul, Leendert C. // Kidney International;May2004, Vol. 65 Issue 5, p1556 

    The pathobiology of chronic allograft nephropathy: Immune-mediated damage and accelerated aging. Chronic allograft nephropathy includes chronic calcineurin nephrotoxicity, recurrent and de novo glomerulonephritis and a group of disorders with graft dysfunction of unknown etiology designated...

  • Antiserum against tumor necrosis factor-alpha and a protease inhibitor reduce immune glomerular injury. Hruby, Zbigniew W.; Shirota, Kinji; Jothy, Serge; Lowry, Robin P. // Kidney International;Jul1991, Vol. 40 Issue 1, p43 

    Previous studies in this laboratory have documented tumor necrosis factor alpha (TNF) release by macrophage laden glomeruli in the accelerated autologous form of nephrotoxic serum nephritis (AA-NTSN). We now report that the administration of anti-TNF antiserum to rats with the AA-NTSN reduces...

  • Glomerular deposition of complement-control proteins in acute and chronic glomerulonephritis. Wyatt, Robert J.; McAdams, A. James; Forristal, Judith; Snyder, Jean; West, Clark D. // Kidney International;Oct1979, Vol. 16 Issue 4, p505 

    Acute poststreptococcal glomerulonephritis (AGN) differed from membranoproliferative glomerulonephritis (MPGN) and lupus nephritis (SLE) in that two of the proteins that control the C3b-dependent convertase. β1H and the C3bC4b-inactivator cofactor (C3bC4blCo), were frequently absent from the...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics