Effects of Folate Supplementation on Two Provisional Molecular Markers of Colon Cancer: A Prospective, Randomized Trial

Young-In Kim; Hyun Wook Baik; Fawaz, Karim; Knox, Tamsin; Young Mee; Norton, Richard; Libby, Eric; Mason, Joel B
January 2001
American Journal of Gastroenterology;Jan2001, Vol. 96 Issue 1, p184
Academic Journal
OBJECTIVES: Dietary folate intake is Inversely associated with the risk of colorectal cancer. This study investigated the effect of folate supplementation on genomic DNA methylation and DNA strand breaks in exons 5-8 of the p53 gene of the colonic mucosa. two provisional biomarkers of colon cancer. METHODS: Twenty subjects with adenomas were randomized to receive either folate (5 mg/day) or placebo for 1 yr after polypectomy. At baseline, 6 months and 1 yr, systemic and colonic measures of folate status were determined, as were the biomarkers mentioned earlier. RESULTS: Folate supplementation increased serum, red blood cell and colonic mucosal folate concentrations (p < 0.02). Folate supplementation also increased the extent of genomic DNA methylation at 6 months and 1 yr (p = O.OO1), whereas placebo administration was associated with an increase in the extent of genomic DNA methylation only at 1 yr. Similarly, folate supplementation decreased the extent of p53 strand breaks in exons 5-8 at 6 months and 1 yr (p < 0.02). whereas placebo administration was associated with a decrease in the extent of p53 strand breaks only at 1 yr. CONCLUSIONS: Both of these provisional biomarkers of colon cancer underwent accelerated improvement at 6 months with folate supplementation. However, these markers also improved with placebo al 1 yr. Therefore, potential confounding factors that seem to modulate these biomarkers need to be identified and corrected in order for these markers to serve as suitable surrogate endpoints in folate chemoprevention trials.


Related Articles

  • Enhancement of Recovery from Psychiatric Illness by Methylfolate. Procter, Andrew // British Journal of Psychiatry;Aug91, Vol. 159, p271 

    ‘41 (33%) of 123 patients with acute psychiatric disorders (DSM Ill diagnosis of major depression or schizophrenia) had borderline or definite folate deficiency (red-cell folate below 200 μg/l) and took part in a double-blind, placebo-controlled trial of methylfolate, 15 mg daily, for 6...

  • Comparison of Fresh Frozen Serum to Traditional Proficiency Testing Material in a College of American Pathologists Survey for Ferritin, Folate, and Vitamin B12. Bock, Jay L.; Endres, David B.; Elin, Ronald J.; Wang, Edward; Rosenzweig, Bruce; Klee, George G. // Archives of Pathology & Laboratory Medicine;Mar2005, Vol. 129 Issue 3, p323 

    Context.—Comparison of different analytical methods in proficiency surveys may be affected by the artificial nature of the survey material. Objective.—To compare intermethod differences in proficiency survey results between 2 types of survey material, conventional proficiency...

  • Low folate levels may protect against colorectal cancer. van Guelpen, B.; Hultdin, J.; Johansson, I.; Hallmans, G.; Stenling, R.; Riboli, E.; Winkvist, A.; Palmqvist, R. // Gut;Oct2006, Vol. 55 Issue 10, p1461 

    Background and aims: Dietary folate is believed to protect against colorectal cancer (CRC). However, few studies have addressed the role of circulating levels of folate. The aim of this study was to relate prediagnostic plasma folate and homocysteine concentrations and the...

  • Fight Cancer "Down There". McCord, Holly; McVeigh, Gloria // Prevention;Apr2003, Vol. 55 Issue 4, p61 

    Getting the highest amount of folate from foods slashed colon cancer risk in men and women and rectal cancer in men by 30%, according to a 7-year study of more than 120,000 people (Cancer, Oct 2002). The colon and rectum are the third most common sites for new tumors, and these cancers rank...

  • Anticancer superheroes. McVeigh, Gloria // Prevention;Sep2004, Vol. 56 Issue 9, p74 

    Reports on a UCLA study on 1,000 people who had had colorectal polyps removed. Finding that those who had more folate in their diets had a lower chance of recurrence; Observation that adequate vitamin B6 must be present for folate to fight cancer; Advantages of high levels of vitamin B6; Advice...

  • Folate intakes and folate biomarker profiles of pregnant Japanese women in the first trimester. Mito, N.; Takimoto, H.; Umegaki, K.; Ishiwaki, A.; Kusama, K.; Fukuoka, H.; Ohta, S.; Abe, S.; Yamawaki, M.; Ishida, H.; Yoshiike, N. // European Journal of Clinical Nutrition;Jan2007, Vol. 61 Issue 1, p83 

    Objective:To assess the status of dietary folate intake, serum and red blood cell (RBC) folate, and related nutritional biomarkers in healthy Japanese women in early pregnancy.Design:A cross-sectional, observational study.Subjects:Pregnant women in the first trimester, at 7–15 weeks...

  • Red cell or serum folate? Results from the National pathology Alliance benchmarking review. Galloway, M.; Rushworth, L. // Journal of Clinical Pathology;Dec2003, Vol. 56 Issue 12, p924 

    Aims: The National pathology Alliance benchmarking review has completed five years of data collection and analysis of the workload and organisation of haematology laboratories in the UK. This study analyses variation in practice in how laboratories respond to a request to determine whether or...

  • Hearty meals.  // U.S. News & World Report;7/08/96, Vol. 121 Issue 2, p76 

    Mentions a report in the `Journal of the American Medical Association' (June 1996) which suggests eating more of citrus, green leafy vegetables and other produce rich in folate. Research which suggests that folate deficiency could trigger heart attack and stroke.

  • piritrexim:. Huber, Jeffrey T.; Gillaspy, Mary L. // Encyclopedic Dictionary of AIDS-Related Terminology;2000, p179 

    A definition of the term "piritrexim" is presented, which refers to a folic acid antagonist.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics