Prevalence of GBV-C/HGV-RNA, Virus Genotypes, and Anti-E2 Antibodies in Autoimmune Hepatitis

Tribl, Barbara; Schöniger-Hekele, Maximilian; Petermann, Dagmar; Bakos, Silvia; Penner, Edward; Müller, Christian
November 1999
American Journal of Gastroenterology;Nov1999, Vol. 94 Issue 11, p3336
Academic Journal
OBJECTIVE: We investigated the prevalence of hepatitis G-RNA (GBV-C/HGV-RNA). a recently cloned new flavivirus. and of antibodies to the envelope 2 antigen (anti-E2), a marker of past infection, in patients with autoimmune hepatitis, and compared it with the prevalence in patients with chronic viral hepatitis and healthy control individuals. METHODS: Sera of 63 patients with autoimmune hepatitis were studied for the presence of GBV-C/HGV-RNA by reverse-transcription polymerase chain reaction and for anti-E2 by enzyme-linked immunosorbent assay. GBV-C/HGV genotypes were determined by genome sequencing. RESULTS: Patients with autoimmune hepatitis had a similar high prevalence of GBV-C/HGV-RNA and anti-E2 antibodies as patients with chronic viral hepatitis B or C. GBV-C/ HGV-RNA was found significantly more often in patients with autoimmune hepatitis (11%, p = 0.045), hepatitis B (16%, p = 0.004), or hepatitis C (21%, p = 0.001) than in healthy controls (2%). The prevalence of anti-E2 antibodies in patients with autoimmune hepatitis was not different from healthy controls (17% vs 13%, NS). The various subtypes of autoimmune hepatitis had similar prevalence rates of GBV-C/ HGV-RNA as patients with liver-kidney microsomal antibody-positive hepatitis C. All of our anti-E2+ (GBV-C/HGV-RNA-) patients were positive for anti-smooth-muscle antibody, whereas only 29% of GBV-C/HGV-RNA+ (anti-E2-) patients were positive (p = 0.025). All seven of the GBV-C/HGV-RNA+ patients with autoimmune hepatitis had genotype 2a, which is also the most prevalent genotype in our region. CONCLUSION: The prevalence of GBV-C/HGV-RNA is significantly increased in patients with autoimmune hepatitis. compared with healthy controls, and is similar to the in-creased prevalence seen in chronic hepatitis B or C patients. Anti-E2 positivity was associated with antibodies against smooth-muscle antigen in all cases. All GBV-C/HGV + autoimmune hepatitis patients were infected with genotype 2a.


Related Articles

  • A Target for Type 3 Autoimmune Hepatitis. McCashland, T. M.; Thompson, Jon S. // American Journal of Gastroenterology;Oct2000, Vol. 95 Issue 10, p2685 

    Discusses research being done on antigens for type 3 auto immune hepatitis. Reference to a study by I. Wies, S. Brunner, J. Henninger et al., published in the 2000 issue of the "Lancet"; Classification of the auto immune hepatitis; Relationship of the pathogenesis of autoimmune hepatitis with...

  • Enzymes as Target Antigens of Liver-Specific Autoimmunity: The Case of Cytochromes P450s. Bogdanos, D. P.; Dalekos, G. N. // Current Medicinal Chemistry;2008, Vol. 15 Issue 22, p2285 

    Characterization of liver-specific autoantigens has given a fresh impetus to research in the pathogenesis of autoimmune liver diseases, viral-triggered and drug-induced autoimmunity affecting the liver. Intriguing is the fact that most of the liver-specific autoantigens are enzymes of key...

  • Interaction of immune complexes isolated from hepatitis C virus-infected individuals with human cell lines. Marino, Rafael; Deibis, Leopoldo; Sanctis, Juan; Bianco, Nicolas; Toro, Felix // Medical Microbiology & Immunology;Jan2005, Vol. 194 Issue 1/2, p73 

    We investigated the interaction of immune complexes (IC) isolated from hepatitis C virus (HCV)-infected individuals with several cell lines that differentially express Fc receptors, and analyzed viral infection by the presence of HCV RNA sequences. Monocytic (U937 and Monomac-6) and lymphocytic...

  • Exploring the Role of Killer Cell Immunoglobulin-Like Receptors and Their HLA Class I Ligands in Autoimmune Hepatitis. Littera, Roberto; Chessa, Luchino; Onali, Simona; Figorilli, Francesco; Lai, Sara; Secci, Luca; La Nasa, Giorgio; Caocci, Giovanni; Arras, Marcella; Melis, Maurizio; Cappellini, Sara; Balestrieri, Cinzia; Serra, Giancarlo; Conti, Maria; Zolfino, Teresa; Casale, Michele; Casu, Stefania; Pasetto, Maria Cristina; Barca, Lucia; Salustro, Claudia // PLoS ONE;1/8/2016, Vol. 11 Issue 1, p1 

    Background: Natural killer cells are involved in the complex mechanisms underlying autoimmune diseases but few studies have investigated their role in autoimmune hepatitis. Killer immunoglobulin-like receptors are key regulators of natural killer cell-mediated immune responses. Methods and...

  • Autoimmune systemic diseases.  // Nephrology Dialysis Transplantation;May2012 Supplement 2, Vol. 27 Issue suppl_2, pii61 

    No abstract available.

  • Autoantibodies to ICA12 (SOX-13) are not specific for Type I diabetes. Steinbrenner, H.; Lohmann, T.; Ostendorf, B.; Scherbaum, W. A.; Seissler, J. // Diabetologia;Nov2000, Vol. 43 Issue 11, p1381 

    Aims/hypothesis. The majority of patients with Type I (insulin-dependent) diabetes mellitus has autoantibodies to insulin, glutamic acid decarboxylase and the tyrosine phosphatase-like protein IA-2. Some patients with Type I diabetes, in particular with adult onset diabetes, have, however, only...

  • Newborn humans manifest autoantibodies to defined self molecules detected by antigen microarray informatics. Merbl, Yifat; Zucker-Toledano, Merav; Quintana, Francisco J.; Cohen, Irun R. // Journal of Clinical Investigation;Mar2007, Vol. 117 Issue 3, p712 

    Autoimmune diseases are often marked by autoantibodies binding to self antigens. However, many healthy persons also manifest autoantibodies that bind to self antigens, known as natural autoantibodies. In order to characterize natural autoantibodies present at birth, we used an antigen microarray...

  • Multiple endometrial antigens are targeted in autoimmune endometriosis. Gajbhiye, R.; Suryawanshi, A.; Khan, S.; Meherji, P.; Warty, N.; Raut, V.; Chehna, N.; Khole, V. // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Jun2008, Vol. 16 Issue 6, p817 

    Endometriosis is defined as the growth of endometrial glands and stroma in ectopic locations. Its aetiology is multifactorial, but autoimmunity has been shown to play a role in its onset and development. The present study aimed to investigate the presence of both IgG and IgM anti-endometrial...

  • Extracellular Domain of Pemphigus Vulgaris Antigen (Desmoglein 3) Mediates Weak Homophilic Adhesion. Amagai, Masayuki; Kàrpàti, Sarolta; Klaus-Kovtun, Vera; Udey, Mark C.; Stanley, John R. // Journal of Investigative Dermatology;Oct94, Vol. 103 Issue 4, p609 

    Pemphigus vulgaris antigen is in the cadherin supergene family. We hypothesized that the extracellular domain of pemphigus vulgaris antigen might mediate homophilic cell adhesion because 1) the originally described cadherins (e.g., E-cadherin) mediate this type of adhesion, 2) pemphigus vulgaris...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics