TITLE

Implication of NF-κB in Helicobacter pylori-Associated Gastritis

AUTHOR(S)
Isomoto, Hajime; Mizuta, Yohei; Miyazaki, Masanobu; Takeshima, Fuminao; Omagari, Katsuhisa; Murase, Kunihiko; Nishiyama, Takashi; Inoue, Ken-ichiro; Murata, Ikuo; Kohno, Shigeru
PUB. DATE
October 2000
SOURCE
American Journal of Gastroenterology;Oct2000, Vol. 95 Issue 10, p2768
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
OBJECTIVE: Transcription factor NF-κB plays a pivotal role in inflammatory responses by up-regulating mRNA expression of bioactive molecules such as chemokines and adhesion molecules. The present study was designed to elucidate the implication of NF-κB in Helicobacter pylori-associated gastritis (HAG). METHODS: We examined 41 patients with HAG and 18 H. pylori-negative control subjects. Expression of activated NF-κB was studied in situ by immunohistochemistry using α-p65 mouse monoclonal antibody (α-p65 mAb), which recognizes activated NF-κB. To identify the cell types in which NF-κB was activated, we performed immunohisto-chemical analysis using antibodies against vascular endothelial cells, macrophages, and B and T lymphocytes. We also examined the colocalization of activated NF-κB with the expression of intercellular adhesion molecule-1 (ICAM-I) on endothelial cells. We measured the levels of NF-κB-dependent chemokines including interleukin-8 (lL-8) and monocyte chemoattractant protein-1 (MCP-1), regulated on activation normal T-cell expressed and secreted (RANTES) and macrophage inflammatory protein-lα (M1P-lα) in antral mucosa by ELISA (ELISA). RESULTS: Activated NF-κB was detected in the nuclei of epithelial cells in antral mucosa, especially of patients with HAG. NF-κB positivity index (NF-κB PI), representing the percentages of epithelial cells with positive nuclear staining for activated NF-κB, was significantly higher in patients with HAG than in H. pylori-negative controls. NF-κB PI correlated significantly with histological scores of gastritis. Moreover, activated NF-κB was identified in the nuclei of vascular endothelial cells, macrophages, and B lymphocytes within the lamina propria in HAG. Colocalization of activated NF-κB with ICAM-1 expression in the same endothelial cells was demonstrated. The IL-8 levels significantly correlated with the NF-κB PI. CONCLUSIONS: In addition to epithelial cells, macrophages, vascular endothelial cells, and B lymphocytes contained activated NF-κB. In these cells, activated NF-κB may be involved in the inflammation process in HAG through the up-regulation of chemokines or adhesion molecules.
ACCESSION #
17623962

 

Related Articles

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics